• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>British Journal of Cancer >Distribution of Photofrin between tumour cells and tumour associated macrophages.
【2h】

Distribution of Photofrin between tumour cells and tumour associated macrophages.

机译：Photofrin在肿瘤细胞和肿瘤相关巨噬细胞之间的分布。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Photofrin levels in cells derived from SCCVII tumours, excised from mice that previously received the drug, were measured using a fluorescence activated cell sorter (FACS). Concomitantly, in the same cells the FACS was used to measure fluorescein isothiocyanate (FITC) fluorescence that originated from FITC-conjugated antimouse IgG added to the cell suspension before sorting. This later measurement enabled discrimination between IgG negative tumour malignant cells and IgG positive host cells (primarily macrophages). In addition, cellular Photofrin content in 'tumour' and 'host' cells sorted by FACS was determined by chemical extraction. The measurements were performed for the time intervals 1-96 h post Photofrin administration. The data showed consistently higher Photofrin levels in the 'host cells', i.e., tumour associated macrophages (TAM), than in 'tumour' cells. On a per cell basis, at any time point studied there was a minimum of 1.7 times more Photofrin in 'host' than in 'tumour cells', while at 4-12 h postadministration, ratios of up to 3.0 times were observed. This corresponds to ratio values greater than 9, when based on Photofrin content per micrograms cell protein.
机译：使用荧光激活细胞分选仪（FACS）测量从SCCVII肿瘤衍生的细胞中的光敏蛋白水平，该细胞是从先前已接受该药物的小鼠中切除的。伴随地，在同一细胞中，FACS用于测量异硫氰酸荧光素（FITC）荧光，该荧光源自分选前添加到细胞悬液中的FITC共轭抗小鼠IgG。此后的测量能够区分IgG阴性肿瘤恶性细胞和IgG阳性宿主细胞（主要是巨噬细胞）。另外，通过化学提取确定通过FACS分选的“肿瘤”和“宿主”细胞中的细胞Photofrin含量。在Photofrin给药后1-96小时的时间间隔内进行测量。数据显示，与“肿瘤”细胞相比，“宿主细胞”即肿瘤相关巨噬细胞（TAM）中的Photofrin水平始终较高。在每个细胞的基础上，在研究的任何时间点，“宿主”中的光敏素最少比“肿瘤细胞”中的光敏素多1.7倍，而在给药后4-12小时，观察到的比率高达3.0倍。当基于每微克细胞蛋白的Photofrin含量时，这对应于大于9的比率值。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号