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首页> 美国卫生研究院文献>British Journal of Cancer >Flow cytometric DNA measurement and cytomorphometric analysis of formalin fixed rat mammary tumours.
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Flow cytometric DNA measurement and cytomorphometric analysis of formalin fixed rat mammary tumours.

机译：福尔马林固定大鼠乳腺肿瘤的流式细胞术DNA测量和细胞形态分析。

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Archival paraffin embedded material was used to examine whether additional quantitative criteria would be helpful to discriminate between histologically benign and malignant rat mammary tumours. To this end nuclear DNA content expressed as DNA ploidy index (DI) was measured using flow cytometry (FCM). A total of 63 benign and malignant mammary tumours were investigated. Thirteen out of 38 (34%) mammary carcinomas were DNA aneuploid against 0 out of 25 benign mammary tumours. Aneuploidy was not significantly increased in tumours showing histological signs of greater malignancy such as cribriform-comedo type or invasive growth. In addition to DI other quantitative criteria indicative for malignancy, such as mitotic count and nuclear morphometric characteristics, were estimated in 24 benign and malignant tubulopapillary tumours, a category where the histological classification may be difficult. It appeared that five out of nine noninvasive tubulopapillary carcinomas and six out of seven invasive carcinomas had abnormal values for either DI, mitotic count or nuclear area or for a combination of these parameters. Each single parameter however was abnormal only in a minority of the malignant tumours. In this respect our data are in accordance with the fact that rat mammary carcinomas are clinically and histologically less malignant than their human counterparts.
机译：档案石蜡包埋材料用于检查其他定量标准是否有助于区分组织学上良性和恶性的大鼠乳腺肿瘤。为此，使用流式细胞仪（FCM）测量以DNA倍性指数（DI）表示的核DNA含量。共调查了63例乳腺良恶性肿瘤。 38个乳腺癌中有13个（34％）是DNA非整倍体，而25个良性乳腺肿瘤中有0个是DNA非整倍体。在表现出更大的恶性组织学征象（如筛状粉刺型或侵袭性生长）的肿瘤中，非整倍性没有显着增加。除了DI以外，还对24种良性和恶性微管乳头状瘤（其组织学分类可能较困难的类别）中的其他恶性肿瘤定量指标进行了评估，例如有丝分裂计数和核形态特征。似乎在9种非侵入性微管乳头状癌中，有5种在7种浸润性癌中有6种具有DI，有丝分裂计数或核面积或这些参数组合的异常值。但是，每个参数仅在少数恶性肿瘤中是异常的。在这方面，我们的数据符合以下事实：大鼠乳腺癌在临床和组织学上的恶性程度低于人类乳癌。

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