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首页> 美国卫生研究院文献>British Journal of Cancer >Rapid detection cloning and molecular cytogenetic characterisation of sequences from an MRP-encoding amplicon by chromosome microdissection.
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Rapid detection cloning and molecular cytogenetic characterisation of sequences from an MRP-encoding amplicon by chromosome microdissection.

机译：通过染色体显微切割对MRP编码扩增子进行序列的快速检测克隆和分子细胞遗传学表征。

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Chromosome microdissection was utilised for the analysis of cytogenetic markers of gene amplification [homogeneously staining regions (hsrs) and double minutes (dmins)] in two doxorubicin-resistant cell lines, fibrosarcoma HT1080/DR4 and small-cell lung cancer H69AR. Microdissection products from the hsr(7)(p12p15) of HT1080/DR4 were amplified and used for fluorescent in situ hybridisation (micro-FISH) analysis of drug-sensitive HT1080, resistant HT1080/DR4 and normal lymphocytes. The results demonstrated that the hsr contains a domain of DNA amplification of complex origin including sequences derived from 16p11.2-16p13.1, 2q11.2, 7q32-7q34 and 10q22. The amplification was confirmed by converting the micro-dissected probe into a microclone library for probing HT1080 and HT1080/DR4 Southerns. A micro-FISH probe from normal band region 16p11-16p13 further demonstrated amplification of 16p sequences in both HT1080/DR4 and H69AR. During the course of this analysis, Cole et al. (1992) (Science, 258, 1650-1653) published the amplification of the MRP gene in H69AR cells, which maps to chromosome 16p13.1. Our results corroborate the finding of MRP amplification in these doxorubicin-resistant cell lines, but, importantly, they provide information on the composition of the complex amplicon contributions from four different chromosomes. This study demonstrates the potential utility of chromosome microdissection for the rapid recovery of sequences from amplified regions in drug-resistant cells.
机译：利用染色体显微解剖技术分析了两个对阿霉素耐药的细胞系纤维肉瘤HT1080 / DR4和小细胞肺癌H69AR中基因扩增的细胞遗传学标记[均染区域（hsrs）和双分钟（dmins）]。 HT1080 / DR4的hsr（7）（p12p15）的显微解剖产物被扩增，并用于对药物敏感的HT1080，耐药性HT1080 / DR4和正常淋巴细胞的荧光原位杂交（micro-FISH）分析。结果表明，hsr包含复杂来源的DNA扩增域，其中包括源自16p11.2-16p13.1、2q11.2、7q32-7q34和10q22的序列。通过将显微切割的探针转换为用于检测HT1080和HT1080 / DR4 Southerns的微克隆文库来确认扩增。来自正常条带区域16p11-16p13的micro-FISH探针进一步证明了HT1080 / DR4和H69AR中16p序列的扩增。在分析过程中，Cole等人。 （1992）（Science，258，1650-1653）发表了MRP基因在H69AR细胞中的扩增，该基因映射到16p13.1染色体。我们的结果证实了在这些对阿霉素耐药的细胞系中MRP扩增的发现，但重要的是，它们提供了有关来自四个不同染色体的复杂扩增子成分组成的信息。这项研究证明了染色体显微切割技术可从耐药细胞中的扩增区域快速恢复序列。

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