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首页> 美国卫生研究院文献>British Journal of Cancer >Interaction between three subpopulations of Ehrlich carcinoma in mixed solid tumours in nude mice: evidence of contact domination.
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Interaction between three subpopulations of Ehrlich carcinoma in mixed solid tumours in nude mice: evidence of contact domination.

机译：裸鼠混合实体瘤中埃氏癌三个亚群之间的相互作用：接触控制的证据。

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Clonal interaction between three subpopulations of Ehrlich carcinoma were studied during growth as mixed solid tumours and as ascites tumours in immune-incompetent nude NMRI mice. The tumour cell lines differed in DNA content as determined by DNA flow cytometry (FCM). Tumour growth was evaluated by tumour growth curves including calculation of tumour volume doubling times, tumour weight on day 14, cell cycle times (per cent labelled mitoses) and cell cycle distributions (FCM). Two subpopulations (E1.15 and E1.95) showed nearly identical growth characteristics during both solid and ascites tumour growth. The third subpopulation (E1.80) grew more slowly. FCM on fine-needle tumour aspirates was used to determine the relative proportions of the cell populations in mixed solid tumours in which E1.95 showed a growth-dominating effect on E1.15. No such effect was demonstrated during single-cell tumour growth in ascitic fluid in which the cells had no intimate contact. Ascitic fluid from E1.95-bearing animals or radiation-killed E1.95 cells had no effect on the growth of E1.15, and no remote effect was seen when the two cell lines were growing in opposite flanks. This indicates that only viable E1.95 cells in close in vivo contact were able to induce growth inhibition of the E1.15 subpopulation. Both the E1.95 and the E1.15 cells dominated the E1.80 cells, but in these cases cell kinetic differences may have played a role as the E1.95 and the E1.15 lines grew faster than the E1.80. The E1.80 cell line had no dominating effect on the E1.15 or E1.95. It is concluded that non-immunologically mediated cellular dominance in heterogeneous tumours may contribute to the evolution of these tumours and may be involved in fundamental tumour biological phenomena.
机译：在免疫功能不全的裸NMRI小鼠中，以混合实体瘤和腹水瘤的形式研究了Ehrlich癌三个亚群之间的克隆相互作用。通过DNA流式细胞术（FCM）确定，肿瘤细胞系的DNA含量不同。通过肿瘤生长曲线评估肿瘤生长，包括计算肿瘤体积加倍时间，第14天的肿瘤重量，细胞周期时间（标记的有丝分裂百分比）和细胞周期分布（FCM）。在实体和腹水肿瘤生长期间，两个亚群（E1.15和E1.95）显示出几乎相同的生长特征。第三亚群（E1.80）的增长速度较慢。 FCM细针肿瘤抽吸物用于确定混合实体瘤中细胞群的相对比例，其中E1.95对E1.15显示出主要的生长作用。在没有紧密接触的腹水的单细胞肿瘤生长过程中，没有发现这种效应。来自带有E1.95的动物的腹水或经辐射杀灭的E1.95细胞对E1.15的生长没有影响，并且当两个细胞系在相反的侧面生长时，没有观察到遥远的影响。这表明，仅活体内密切接触的活E1.95细胞能够诱导E1.15亚群的生长抑制。 E1.95和E1.15细胞都主导着E1.80细胞，但在这些情况下，细胞动力学差异可能起了作用，因为E1.95和E1.15系的生长速度快于E1.80。 E1.80细胞系对E1.15或E1.95没有主导作用。结论是异质性肿瘤中非免疫学介导的细胞优势可能有助于这些肿瘤的进化，并可能参与基本的肿瘤生物学现象。

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