• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>British Journal of Cancer >The presence of circulating total DNA and methylated genes is associated with circulating tumour cells in blood from breast cancer patients
【2h】

The presence of circulating total DNA and methylated genes is associated with circulating tumour cells in blood from breast cancer patients

机译：循环总DNA和甲基化基因的存在与乳腺癌患者血液中循环的肿瘤细胞有关

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Circulating tumour cells (CTC) and tumour-related methylated DNA in blood have been separately assessed for their utility as a marker for subclinical metastasis in breast cancer. However, no studies have looked into the relation between the both molecular markers in this type of cancer. In this study, we investigated the correlations between total/methylated DNA and CTC in the blood from metastatic breast cancer patients. We simultaneously obtained whole blood, plasma and serum samples from 80 patients and 20 controls. The CellSearch System was used to enumerate CTC in blood samples. Plasma total DNA levels were determined by a QPCR method. Sera were analysed by methylation-specific QPCR for three markers: adenomatous polyposis coli (APC), ras association domain family protein 1A (RASSF1A) and oestrogen receptor 1 (ESR1). Total DNA levels in patients were significantly increased when compared with controls (P<0.001) and correlated with the number of CTC (r=0.418, P<0.001). Hypermethylation of one or more genes was detected in 42 (53%) serum samples from breast cancer patients and in three (16%) serum samples from controls (P=0.003). APC was hypermethylated in 29%, RASSF1A in 35% and ESR1 in 20% of breast cancer cases. Detection of a methylated gene in serum was associated with the detection of CTC in blood (P=0.03). The detection of large amounts of circulating total/methylated DNA correlated with the presence of CTC in the blood from patients with breast cancer. This can be interpreted in two ways: (a) CTC are a potential source of circulating tumour-specific DNA; (b) high numbers of CTC and circulating methylated DNA are both a phenotypic feature of more aggressive tumour biology.
机译：血液中的循环肿瘤细胞（CTC）和肿瘤相关的甲基化DNA已被分别评估作为乳腺癌亚临床转移标志物的效用。但是，尚无研究探讨这种类型癌症中两种分子标记之间的关系。在这项研究中，我们调查了转移性乳腺癌患者血液中总甲基化DNA与CTC之间的相关性。我们同时从80位患者和20位对照中获得了全血，血浆和血清样本。 CellSearch系统用于计数血液样本中的四氯化碳。通过QPCR方法测定血浆总DNA水平。通过甲基化特异性QPCR分析血清中的三种标志物：腺瘤性息肉病（APC），ras缔合域家族蛋白1A（RASSF1A）和雌激素受体1（ESR1）。与对照组相比，患者的总DNA水平显着增加（P <0.001），并与CTC数量相关（r = 0.418，P <0.001）。在来自乳腺癌患者的42个（53％）血清样本和来自对照的三个（16％）血清样本中检测到一种或多种基因的超甲基化（P = 0.003）。在乳腺癌病例中，APC甲基化程度高达29％，RASSF1A为35％，ESR1为20％。血清中甲基化基因的检测与血液中CTC的检测相关（P = 0.03）。乳腺癌患者血液中大量循环总甲基化DNA的检测与CTC的存在有关。这可以用两种方式来解释：（a）CTC是循环肿瘤特异性DNA的潜在来源； （b）大量的四氯化碳和循环甲基化的DNA都是更具侵略性的肿瘤生物学的表型特征。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号