• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>The Journal of Physiology >Cell content of phosphatidylinositol (45)bisphosphate in Ehrlich mouse ascites tumour cells in response to cell volume perturbations in anisotonic and in isosmotic media
【2h】

Cell content of phosphatidylinositol (45)bisphosphate in Ehrlich mouse ascites tumour cells in response to cell volume perturbations in anisotonic and in isosmotic media

机译：Ehrlich小鼠腹水肿瘤细胞中磷脂酰肌醇（45）二磷酸的细胞含量对等渗和等渗介质中细胞体积扰动的响应

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The labelling pattern of cellular phosphoinositides (PtdInsPn) was studied in Ehrlich ascites cells labelled in vivo for 24 h with myo-[2-3H]- or l-myo-[1-3H]inositol and exposed to anisotonic or isosmotic volume perturbations. In parallel experiments the cell volume ([14C]3-OMG space) was monitored. In hypotonic media the cells initially swelled osmotically and subsequently as expected showed a regulatory volume decrease (RVD) response. Concurrently, the cell content of PtdInsP2 showed a marked, transient decrease and the content of PtdInsP a small, transient increase. The changes in PtdInsP2 and PtdInsP content increased progressively with the extent of hypotonicity (in the range 1.00–0.50 relative osmolarity). No evidence was found for either hydrolysis of PtdInsP2 or formation of PtdInsP3. In hypertonic medium (relative osmolarity 1.50), cells initially shrank osmotically and subsequently as expected showed a small regulatory volume increase (RVI) response. Concurrently, the cell content of PtdInsP2 showed a marked increase and the content of PtdInsP a small decrease, i.e. changes in the opposite direction of those seen in hypotonic media. In isosmotic media with high (100 mm) or low (0.8 mm) K+ concentration, cells slowly swelled or shrank due to uptake or loss of isosmotic KCl. Under these conditions, with largely unchanged intracellular ionic strength, the cell content of PtdInsP2 and PtdInsP remained constant. Our results show that PtdInsP2 is not volume sensitive per se, and moreover that the regulatory volume adjustments in Ehrlich ascites cells are not mediated by PtdInsP2 hydrolysis and its subsequent production of second messengers. The simplest interpretation of the observed effects would be that PtdInsP2 is controlled by ionic strength, probably via activation/inhibition of phosphoinositide-specific phosphatases/kinases. In Ehrlich ascites cells, as shown previously, the opposing ion channels and transporters activated during RVD and RVI, respectively, are controlled with tight negative coordination by a common cell volume ‘set-point’ that is shifted in anisotonic media, but unchanged during cell swelling in isosmotic high K+ medium. We hypothesize that PtdInsP2 might orchestrate this ‘set-point’ shift.
机译：研究了在体内用myo- [2- 3 H]-或l-myo- [1- 3标记的Ehrlich腹水细胞中细胞磷酸肌醇（PtdInsPn）的标记模式 H]肌醇并暴露于等渗或等渗的体积扰动中。在平行实验中，监测细胞体积（[ 14 C] 3-OMG空间）。在低渗介质中，细胞最初渗透性溶胀，随后如预期的那样显示出调节体积减少（RVD）反应。同时，PtdInsP2的细胞含量显示出明显的瞬时降低，而PtdInsP的含量则显示出短暂的升高。 PtdInsP2和PtdInsP含量的变化随着低渗程度的增加而逐渐增加（相对渗透压范围为1.00-0.50）。没有发现水解PtdInsP2或形成PtdInsP3的证据。在高渗介质中（相对渗透压为1.50），细胞最初会发生渗透性收缩，随后如预期的那样会显示出较小的调节体积增加（RVI）反应。同时，PtdInsP2的细胞含量显着增加，PtdInsP的含量略有下降，即与低渗介质所见方向相反。在高（100 mm）或低（0.8 mm）K + 浓度的等渗培养基中，由于等渗KCl的吸收或损失，细胞缓慢溶胀或收缩。在这些条件下，细胞内离子强度基本不变，PtdInsP2和PtdInsP的细胞含量保持恒定。我们的结果表明，PtdInsP2本身对体积不敏感，此外，Ehrlich腹水细胞的调节体积调节不受PtdIns P 2水解及其随后产生第二信使的介导。对观察到的影响的最简单解释是，PtdIns P 2受离子强度的控制，可能是通过磷酸肌醇特异性磷酸酶/激酶的激活/抑制来实现的。如前所示，在Ehrlich腹水细胞中，在RVD和RVI期间分别激活的相对的离子通道和转运蛋白受到共同的细胞体积“设定点”的严格负协调控制，该细胞体积“设定点”在各向异性介质中移动，但在细胞过程中不变在等渗高K + 培养基中溶胀。我们假设PtdIns P 2可能会协调这种“设定点”转换。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号