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首页> 美国卫生研究院文献>BMC Veterinary Research >Prevalence and molecular characterization of Clostridium difficile isolated from feedlot beef cattle upon arrival and mid-feeding period
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Prevalence and molecular characterization of Clostridium difficile isolated from feedlot beef cattle upon arrival and mid-feeding period

机译：肥育肉牛抵达和中期饲养期间分离到的艰难梭菌的流行和分子特征

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BackgroundThe presence of indistinguishable strains of Clostridium difficile in humans, food animals and food, as well as the apparent emergence of the food-animal-associated ribotype 078/toxinotype V as a cause of community-associated C. difficile infection have created concerns about the potential for foodborne infection. While studies have reported C. difficile in calves, studies of cattle closer to the age of harvest are required. Four commercial feedlots in Alberta (Canada) were enrolled for this study. Fecal samples were collected at the time of arrival and after acclimation (< 62, 62-71 or > 71 days on feed). Selective culture for Clostridium difficile was performed, and isolates were characterized by ribotyping and pulsed-field gel electrophoresis. A logistic regression model was built to investigate the effect of exposure to antimicrobial drugs on the presence of C. difficile.
机译：背景在人类，食用动物和食品中难辨梭状芽胞杆菌的存在以及与食物-动物相关的核糖型078 /毒素型V的明显出现是引起社区相关的艰难梭菌感染的原因，这引起了人们的关注。食源性感染的可能性。虽然研究报告了犊牛的艰难梭菌，但仍需要对接近收获年龄的牛进行研究。这项研究招募了艾伯塔省（加拿大）的四个商业饲养场。在到达时和适应后（进食时<62、62-71或> 71天）收集粪便样品。进行艰难梭菌的选择性培养，并通过核糖分型和脉冲场凝胶电泳对分离株进行表征。建立了逻辑回归模型，以研究暴露于抗菌药物对艰难梭菌的影响。

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