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A comparison of real-time PCR and reverse line blot hybridization in detecting feline haemoplasmas of domestic cats and an analysis of risk factors associated with haemoplasma infections

机译：实时荧光定量PCR和反向线杂交法检测家猫猫血友病的比较及与血友病感染相关的危险因素分析。

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摘要

BackgroundThree species of feline haemoplasma are recognised: Mycoplasma haemofelis (Mhf), ‘Candidatus Mycoplasma haemominutum’ (CMhm) and ‘Candidatus Mycoplasma turicensis (CMt). This study compared a reverse line blot hybridization (RLB) assay for simultaneous detection of Mhf, CMhm with three separate quantitative real-time polymerase chain reaction (qPCR) assays used for diagnosis of Mhf, CMhm and CMt. The RLB and qPCR assays were applied to DNA extracted from blood samples collected from 154 cats from Trinidad and Tobago.

机译：背景技术识别出三种血友病猫种：血支原体（Mhf），“血型念珠菌”（CMhm）和“土生支原体”（CMt）。这项研究比较了用于同时检测Mhf，CMhm的反向线印迹杂交（RLB）测定法和用于诊断Mhf，CMhm和CMt的三种单独的定量实时聚合酶链反应（qPCR）测定法。将RLB和qPCR分析应用于从特立尼达和多巴哥的154只猫收集的血液样本中提取的DNA。

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期刊名称 BMC Veterinary Research
作者
Karla Georges; Chuckwudozi Ezeokoli; Tennille Auguste; Nisshi Seepersad; Akua Pottinger; Olivier Sparagano; Séverine Tasker;
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作者单位

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年(卷),期 2012(8),-1
年度 2012
页码 103
总页数 9
原文格式 PDF
正文语种
中图分类动物医学（兽医学）;
关键词
Feline haemoplasmas qPCR Reverse line blot Feline retrovirus infection;

机译：猫血肿;qPCR;反向印迹;猫逆转录病毒感染;

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专利

1. A comparison of real-time PCR and reverse line blot hybridization in detecting feline haemoplasmas of domestic cats and an analysis of risk factors associated with haemoplasma infections [J] . Karla Georges, Chuckwudozi Ezeokoli, Tennille Auguste, BMC Veterinary Research . 2012,第1期

机译：实时荧光定量PCR和反向线杂交技术检测家猫猫血友病的比较及与血友病感染相关的危险因素分析。
2. Prevalence and risk factor analysis of feline haemoplasma infection in New Zealand domestic cats using a real-time PCR assay. [J] . Jenkins K. S., Dittmer K. E., Marshall J. C., Journal of feline medicine and surgery . 2013,第12期

机译：使用实时PCR分析法对新西兰家猫猫血友病感染的患病率和危险因素进行分析。
3. Prevalence of feline haemoplasma infection in cats in Southern Bavaria, Germany, and infection risk factor analysis [J] . Silja Laberke, Frank Just, Kurt Pfister, Berliner und Munchener Tierarztliche Wochenschrift . 2010,第1a2期

机译：德国南部巴伐利亚州猫的血友病感染率及感染危险因素分析
4. Clinical Evaluation of PCR-Reverse Blot Hybridization Assay for Pathogen Identification Using Whole Blood of Patients with Suspected Sepsis [C] . Jungho Kim, Seoyoung Kim, Hye-young Wang, International Molecular Medicine Tri-Conference. . 2016

机译：使用疑似脓毒症患者全血的PCR反转杂交测定对病原体鉴定的临床评价
5. Experimental infection of domestic cats with various feline Bartonella species or types. [D] . Yamamoto, Kazuhiro. 2002

机译：实验性家猫感染各种猫巴尔通体物种或类型。
6. Prevalence and risk factor analysis for feline haemoplasmas in cats from Northern Serbia with molecular subtyping of feline immunodeficiency virus [O] . Elpida Sarvani, Séverine Tasker, Milica Kovacˇević Filipović, 2018

机译：猫免疫缺陷病毒的分子亚型分析：塞尔维亚北部猫血友病的患病率和危险因素分析
7. A comparison of real-time PCR and reverse line blot hybridization in detecting feline haemoplasmas of domestic cats and an analysis of risk factors associated with haemoplasma infections [O] . Karla Georges, Chuckwudozi Ezeokoli, Tennille Auguste, 2012

机译：实时荧光定量PCR和反向线杂交法检测家猫猫血友病的比较及与血友病感染相关的危险因素分析。

A comparison of real-time PCR and reverse line blot hybridization in detecting feline haemoplasmas of domestic cats and an analysis of risk factors associated with haemoplasma infections

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