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DHPLC technology for high-throughput detection of mutations in a durum wheat TILLING population

机译:DHPLC技术用于高通量检测硬质小麦TILLING群体中的突变

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摘要

BackgroundDurum wheat (Triticum turgidum L.) is a cereal crop widely grown in the Mediterranean regions; the amber grain is mainly used for the production of pasta, couscous and typical breads. Single nucleotide polymorphism (SNP) detection technologies and high-throughput mutation induction represent a new challenge in wheat breeding to identify allelic variation in large populations. The TILLING strategy makes use of traditional chemical mutagenesis followed by screening for single base mismatches to identify novel mutant loci. Although TILLING has been combined to several sensitive pre-screening methods for SNP analysis, most rely on expensive equipment. Recently, a new low cost and time saving DHPLC protocol has been used in molecular human diagnostic to detect unknown mutations.
机译:背景硬质小麦(Triticum turgidum L.)是一种在地中海地区广泛种植的谷物作物。琥珀色谷物主要用于生产面食,蒸粗麦粉和典型面包。单核苷酸多态性(SNP)检测技术和高通量突变诱导是小麦育种中鉴定大种群等位基因变异的新挑战。 TILLING策略利用传统的化学诱变方法,然后筛选单碱基错配以鉴定新的突变基因座。尽管将TILLING与几种敏感的预筛选方法结合起来进行SNP分析,但大多数方法都依赖于昂贵的设备。最近,一种新的低成本且省时的DHPLC方案已用于分子人类诊断中以检测未知突变。

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