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Analysis of the bacterial community in chronic obstructive pulmonary disease sputum samples by denaturing gradient gel electrophoresis and real-time PCR

机译：变性梯度凝胶电泳和实时荧光定量PCR分析慢性阻塞性肺疾病痰标本中的细菌群落

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BackgroundThe Global Initiative defines COPD for chronic obstructive lung disease as an entirely preventable and treatable disease characterized by sputum production, bacterial colonisation, neutrophilic bronchial airway inflammation and poor health status. The World Health Organization (WHO) estimates that COPD will become the fourth-most common cause of death worldwide, just behind ischemic heart disease, cerebrovascular disease and HIV/AIDS, by 2030. The aim of this study was to determine the main structure feature of sputum potentially pathogenic microorganisms in subjects with COPD during the clinical stable state.

机译：背景全球行动计划将慢性阻塞性肺疾病的COPD定义为一种完全可预防和治疗的疾病，其特征是痰液产生，细菌定植，嗜中性支气管气道炎症和健康状况不佳。世界卫生组织（WHO）估计，到2030年，COPD将成为全球第四大常见死因，仅次于缺血性心脏病，脑血管疾病和HIV / AIDS。该研究的目的是确定主要结构特征临床稳定状态下COPD患者痰中潜在病原微生物的检测

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期刊名称 BMC Pulmonary Medicine
作者
Dachang Wu; Chenxia Hou; Yanxia Li; Zinan Zhao; Jianjun Liu; Xin Lu; Xueqi Shang; Yi Xin;
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作者单位

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年(卷),期 2014(14),-1
年度 2014
页码 179
总页数 7
原文格式 PDF
正文语种
中图分类外科学;
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专利

1. Analysis of the bacterial community in chronic obstructive pulmonary disease sputum samples by denaturing gradient Gel electrophoresis and real-time PCR [J] . Dachang Wu, Chenxia Hou, Yanxia Li, BMC Pulmonary Medicine . 2014,第1期

机译：变性梯度凝胶电泳和实时荧光定量PCR分析慢性阻塞性肺疾病痰标本中的细菌群落
2. Dynamics of Vaginal Bacterial Communities in Women Developing Bacterial Vaginosis, Candidiasis, or No Infection, Analyzed by PCR-Denaturing Gradient Gel Electrophoresis and Real-Time PCR [J] . Beatrice Vitali, Ciro Pugliese, Elena Biagi, Applied and Environmental Microbiology . 2007,第18期

机译：PCR-变性梯度凝胶电泳和实时荧光定量PCR分析妇女细菌性阴道病，念珠菌病或无感染的妇女阴道细菌群落的动态
3. Bacterial population dynamics and community structure in a pharmaceutical manufacturing water supply system determined by real-time PCR and PCR-denaturing gradient gel electrophoresis [J] . Kawai M, Yamagishi J, Yamaguchi N, Journal of applied microbiology . 2004,第6期

机译：实时PCR和PCR变性梯度凝胶电泳确定的制药生产供水系统中细菌种群动态和群落结构
4. Analysis of bacterial community in membrane-separation bioreactors by fluorescent in situ hybridization (FISH) and denaturing gradient gel electrophoresis (DGGE) techniques [C] . B.S.Luxmy, F.Nakajima, Kazuo Yamamoto 2nd International Conference on Membrane Technology in Environmental Management, held in Tokyo, Japan, 1-4 November 1999 . 1999

机译：通过荧光原位杂交（FISH）和变性梯度凝胶电泳（DGGE）技术分析膜分离生物反应器中的细菌群落
5. Soil community analysis along a salt gradient using denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA genes. [D] . Dutta, Partha. 2006

机译：使用PCR扩增的16S rRNA基因的变性梯度凝胶电泳，沿着盐梯度进行土壤群落分析。
6. Dynamics of Vaginal Bacterial Communities in Women Developing Bacterial Vaginosis Candidiasis or No Infection Analyzed by PCR-Denaturing Gradient Gel Electrophoresis and Real-Time PCR [O] . Beatrice Vitali, Ciro Pugliese, Elena Biagi, 2007

机译：PCR-变性梯度凝胶电泳和实时荧光定量PCR分析妇女细菌性阴道病念珠菌病或无感染的妇女阴道细菌群落的动态
7. Analysis of the bacterial community in chronic obstructive pulmonary disease sputum samples by denaturing gradient gel electrophoresis and real-time PCR [O] . Dachang Wu, Chenxia Hou, Yanxia Li, 2014

机译：变性梯度凝胶电泳和实时荧光定量PCR分析慢性阻塞性肺疾病痰标本中的细菌群落

Analysis of the bacterial community in chronic obstructive pulmonary disease sputum samples by denaturing gradient gel electrophoresis and real-time PCR

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