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Somatic embryogenesis tetraploidy and variant leaf morphology in transgenic diploid strawberry (Fragaria vesca subspecies vesca ‘Hawaii 4’)

机译:转基因二倍体草莓中的体细胞胚发生四倍体和叶片形态变异(草莓vesca亚种vescaHawaii 4)

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摘要

BackgroundThe diploid (2n = 2x = 14) strawberry model plant Fragaria vesca ssp. vesca ‘Hawaii 4’ was employed for functional analysis of expressed DNA sequences initially identified as being unique to Fragaria and of unknown or poorly understood function. ‘Hawaii 4’ is prominent in strawberry research due to its ease of Agrobacterium-mediated transformation and regenerability, and its status as the source of the first complete strawberry genomic sequence. Our studies of a set of transformants have documented intriguing, construct-associated effects on leaf morphology, and provide important and unexpected insights into the performance of the ‘Hawaii 4’ transformation and regeneration system.
机译:背景二倍体(2n = 2x = 14)草莓模型植物草莓(Fragaria vesca ssp)。 vesca'Hawaii 4'用于表达的DNA序列的功能分析,该DNA序列最初被识别为草莓属独有,且功能未知或不为人所知。 “ Hawaii 4”在草莓研究中非常重要,这是因为它易于农杆菌介导的转化和可再生性,并且是第一个完整的草莓基因组序列的来源。我们对一组转化子的研究已经证明了对叶片形态的有趣,与构建相关的作用,并为“夏威夷4号”转化和再生系统的性能提供了重要而出乎意料的见解。

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