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Comparative transcriptome profiling of the fertile and sterile flower buds of a dominant genic male sterile line in sesame (Sesamum indicum L.)

机译:芝麻(Sesamum indicum L.)显性基因雄性不育系的能育和不育花芽的比较转录组分析

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摘要

BackgroundSesame (Sesamum indicum L.) is a globally important oilseed crop with highly-valued oil. Strong hybrid vigor is frequently observed within this crop, which can be exploited by the means of genic male sterility (GMS). We have previously developed a dominant GMS (DGMS) line W1098A that has great potential for the breeding of F1 hybrids. Although it has been genetically and anatomically characterized, the underlying molecular mechanism for male sterility remains unclear and therefore limits the full utilization of such GMS line. In this study, RNA-seq based transcriptome profiling was carried out in two near-isogenic DGMS lines (W1098A and its fertile counterpart, W1098B) to identify differentially expressed genes (DEGs) related to male sterility.
机译:背景技术芝麻(Sesamum indicum L.)是一种全球重要的油料作物,具有高价值的油料。在这种作物中经常观察到强烈的杂种活力,可以通过基因雄性不育(GMS)加以利用。我们先前已经开发了占主导地位的GMS(DGMS)品系W1098A,该品种在F1杂种育种方面具有巨大潜力。尽管已经在遗传和解剖学上进行了表征,但男性不育的潜在分子机制仍然不清楚,因此限制了这种GMS品系的充分利用。在这项研究中,在两个近等基因的DGMS系(W1098A及其可育的对应物W1098B)中进行了基于RNA-seq的转录组分析,以鉴定与雄性不育相关的差异表达基因(DEG)。

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