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High-resolution time series of Pseudomonas aeruginosa gene expression and rhamnolipid secretion through growth curve synchronization

机译:通过生长曲线同步的高分辨率铜绿假单胞菌基因表达和鼠李糖脂分泌的时间序列

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摘要

BackgroundOnline spectrophotometric measurements allow monitoring dynamic biological processes with high-time resolution. Contrastingly, numerous other methods require laborious treatment of samples and can only be carried out offline. Integrating both types of measurement would allow analyzing biological processes more comprehensively. A typical example of this problem is acquiring quantitative data on rhamnolipid secretion by the opportunistic pathogen Pseudomonas aeruginosa. P. aeruginosa cell growth can be measured by optical density (OD600) and gene expression can be measured using reporter fusions with a fluorescent protein, allowing high time resolution monitoring. However, measuring the secreted rhamnolipid biosurfactants requires laborious sample processing, which makes this an offline measurement.
机译:背景技术在线分光光度法测量可以以高分辨率监视动态生物过程。相反,许多其他方法需要费力地处理样品,并且只能离线进行。将两种类型的测量结果整合在一起可以更全面地分析生物过程。此问题的一个典型示例是获取有关机会病原体铜绿假单胞菌鼠李糖脂分泌的定量数据。铜绿假单胞菌的细胞生长可以通过光密度(OD600)进行测量,而基因表达可以通过将报告基因与荧光蛋白融合来进行测量,从而实现高时间分辨率的监测。然而,测量分泌的鼠李糖脂生物表面活性剂需要费力的样品处理,这使其成为离线测量。

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