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An improved assay for rapid detection of viable Staphylococcus aureus cells by incorporating surfactant and PMA treatments in qPCR

机译：通过在qPCR中结合表面活性剂和PMA处理快速检测活的金黄色葡萄球菌细胞的改进检测方法

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BackgroundStaphylococcus aureus is an important human pathogen causing a variety of life-threatening diseases. Rapid and accurate detection of Staphylococcus aureus is a necessity for prevention of outbreaks caused by this pathogen. PCR is a useful tool for rapid detection of foodborne pathogens, however, its inability to differentiate DNA from dead cells and live cells in amplification severely limits its application in pathogen detection. The aim of this study was to develop an improved assay was developed by incorporating the sample treatments with a surfactant and propidium monoazide (PMA) in qPCR for detection of viable S. aureus cells.

机译：背景金黄色葡萄球菌是引起多种威胁生命的疾病的重要人类病原体。快速，准确地检测金黄色葡萄球菌是预防由该病原体引起的暴发的必要条件。 PCR是用于快速检测食源性病原体的有用工具，但是，无法在扩增中将DNA与死细胞和活细胞区分开来，严重限制了其在病原体检测中的应用。这项研究的目的是开发一种改进的测定方法，该方法是通过将样品处理剂与表面活性剂和单叠氮化丙锭（PMA）合并到qPCR中以检测活的金黄色葡萄球菌而开发的。

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期刊名称 BMC Microbiology
作者
Chen Zi; Dexin Zeng; Nan Ling; Jianjun Dai; Feng Xue; Yuan Jiang; Baoguang Li;
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作者单位

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年(卷),期 2018(18),-1
年度 2018
页码 132
总页数 8
原文格式 PDF
正文语种
中图分类微生物学;
关键词
Staphylococcus aureus PMA-qPCR Sarkosyl Triton x-100 Accurate detection Viability False positive Milk Methicillin-resistant Staphylococcus aureus;

机译：金黄色葡萄球菌;PMA-qPCR;Sarkosyl;Triton x-100;准确检测;生存力;假阳性;牛奶;耐甲氧西林金黄色葡萄球菌;

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专利

1. An improved assay for rapid detection of viable Staphylococcus aureus cells by incorporating surfactant and PMA treatments in qPCR [J] . Chen Zi, Dexin Zeng, Nan Ling, BMC Microbiology . 2018,第1期

机译：通过在qPCR中结合表面活性剂和PMA处理，快速检测活的金黄色葡萄球菌细胞的改进检测方法
2. Multiplex PMA-qPCR Assay with Internal Amplification Control for Simultaneous Detection of Viable Legionella pneumophila, Salmonella typhimurium, and Staphylococcus aureus in Environmental Waters [J] . Haiyan Li, Hongyi Xin, Sam Fong Yau Li Environmental Science & Technology . 2015,第24期

机译：带有内部扩增对照的多重PMA-qPCR分析可同时检测环境水域中可行的嗜肺军团菌，鼠伤寒沙门氏菌和金黄色葡萄球菌
3. Rapid and simultaneous detection of viable Cronobacter sakazakii, Staphylococcus aureus, and Bacillus cereus in infant food products by PMA-mPCR assay with internal amplification control [J] . Li Fan, Xie Guoyang, Zhou Baoqing, LWT-Food Science & Technology . 2016,第Null期

机译：通过内部扩增控制的PMA-mPCR快速快速同时检测婴儿食品中可行的阪崎肠杆菌，金黄色葡萄球菌和蜡状芽孢杆菌
4. PathoProof Mastitis PCR Assay and Sensitivity Validation of a New Test Kit for Rapid Identification of Staphylococcus aureus, Streptococcus agalactiae and Mycoplasma bovis From Bovine Milk [C] . M. Silvennoinen, J. Holopainen, M.T. Koskinen Annual Meeting of the National Mastitis Council . 2010

机译：用于快速鉴定金黄色葡萄球菌，链球菌胆碱和支原体Bovis的新试剂盒的乳腺炎乳腺炎PCR测定和敏感性验证
5. Rapid and sensitive detection of aflatoxin in animal feeds and food grains using immunomagnetic bead based recovery and real-time immuno quantitative PCR (RT-iqPCR) assay. [D] . Babu, Dinesh. 2010

机译：使用基于免疫磁珠的回收率和实时免疫定量PCR（RT-iqPCR）分析快速，灵敏地检测动物饲料和粮食中的黄曲霉毒素。
6. Propidium monoazide–quantitative polymerase chain reaction (PMA-qPCR) assay for rapid detection of viable and viable but non-culturable (VBNC) Pseudomonas aeruginosa in swimming pools [O] . Abdolali Golpayegani, Masoumeh Douraghi, Farhad Rezaei, 2019

机译：单叠氮化丙锭定量聚合酶链反应（PMA-qPCR）测定法可快速检测游泳池中有毒和无毒（VBNC）的铜绿假单胞菌
7. Propidium monoazide–quantitative polymerase chain reaction (PMA-qPCR) assay for rapid detection of viable and viable but non-culturable (VBNC) Pseudomonas aeruginosa in swimming pools [O] . Abdolali Golpayegani, Masoumeh Douraghi, Farhad Rezaei, 2019

机译：单叠氮酰胺定量聚合酶链反应（PMA-QPCR）测定，用于快速检测可行和可行但非培养的（VBNC）假单胞菌在游泳池中的铜绿假单胞菌

An improved assay for rapid detection of viable Staphylococcus aureus cells by incorporating surfactant and PMA treatments in qPCR

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