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Identification and characterization of novel and conserved microRNAs in several tissues of the Chinese rare minnow (Gobiocypris rarus) based on illumina deep sequencing technology

机译:基于照明深度测序技术的中国稀有min鱼(Gobiocypris rarus)几个组织中新的和保守的microRNA的鉴定和表征

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摘要

BackgroundMicroRNAs (miRNAs), which comprise a large family of endogenous small non-coding RNA molecules, play important roles in the regulation of gene expression in various biological processes. The Chinese rare minnow (Gobiocypris rarus) is a Chinese native fish species and is used extensively as an experimental fish in China; however, relevant biological data, especially miRNA transcriptome data, have not been well documented. To discover conserved and potential novel miRNAs in Chinese rare minnows, a pool of equal amounts of RNA obtained from 6 different adult rare minnow tissues (brain, eye, gill, liver, muscle and heart) was sequenced using illumina deep sequencing technology.
机译:背景MicroRNA(miRNA)包含大量的内源性非编码小RNA分子家族,在各种生物学过程中的基因表达调控中起着重要作用。中国稀有的min鱼(Gobiocypris rarus)是中国本土鱼类,在中国被广泛用作实验鱼。但是,相关的生物学数据,尤其是miRNA转录组数据,尚未得到很好的记录。为了在中国稀有小鱼中发现保守的和潜在的新型miRNA,使用照明深度测序技术对从6种不同的成年稀有小鱼组织(大脑,眼,腮,肝,肌肉和心脏)中获得的等量RNA进行了测序。

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