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Genetic mapping and comparative genomics to inform restoration enhancement and culture of southern flounder Paralichthys lethostigma

机译:遗传作图和比较基因组学为南部比目鱼Paraliichthys lethostigma的恢复增强和养殖提供信息

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摘要

BackgroundSouthern flounder, Paralichthys lethostigma, historically support a substantial fishery along the Atlantic and Gulf coasts of the southern United States. Low year-class strengths over the past few years in the western Gulf of Mexico have raised concern that spawning stocks may be overfished. Current management of the resource includes releasing hatchery-raised juveniles to restock bays and estuaries; additionally, there is a growing interest in the potential for commercial aquaculture of the species. Currently, genomic resources for southern flounder do not exist. Here, we used two hatchery-reared families and double-digest, restriction-site-associated DNA (ddRAD) sequencing to create a reduced-representation genomic library consisting of several thousand single nucleotide polymorphisms (SNPs) located throughout the genome.
机译:背景技术历史上,南部比目鱼Paralichthys lethostigma支持美国南部大西洋和墨西哥湾沿岸的大量渔业。在过去的几年中,墨西哥湾西部地区的年级实力低下,引起了人们对产卵种群可能被过度捕捞的担忧。当前对资源的管理包括将孵化场饲养的幼体释放到补给海湾和河口;此外,人们对该物种商业化水产养殖的潜力越来越感兴趣。当前,南部比目鱼的基因组资源不存在。在这里,我们使用了两个孵化场饲养的家族和双重消化的,限制性位点相关的DNA(ddRAD)测序,创建了一个具有代表性的基因组文库,该文库由位于整个基因组的数千个单核苷酸多态性(SNP)组成。

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