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Expanding anchored hybrid enrichment to resolve both deep and shallow relationships within the spider tree of life

机译:扩展锚定混合富集以解决蜘蛛生命树中的深层和浅层关系

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摘要

BackgroundDespite considerable effort, progress in spider molecular systematics has lagged behind many other comparable arthropod groups, thereby hindering family-level resolution, classification, and testing of important macroevolutionary hypotheses. Recently, alternative targeted sequence capture techniques have provided molecular systematics a powerful tool for resolving relationships across the Tree of Life. One of these approaches, Anchored Hybrid Enrichment (AHE), is designed to recover hundreds of unique orthologous loci from across the genome, for resolving both shallow and deep-scale evolutionary relationships within non-model systems. Herein we present a modification of the AHE approach that expands its use for application in spiders, with a particular emphasis on the infraorder Mygalomorphae.
机译:背景技术尽管付出了巨大的努力,但蜘蛛分子系统学的研究却落后于许多其他可比的节肢动物群,从而阻碍了重要的宏观进化假说的家族水平的解析,分类和检验。最近,替代性靶向序列捕获技术为分子系统学提供了解决生命之树中各种关系的强大工具。这些方法之一是锚定杂交富集(AHE),旨在从整个基因组中回收数百个独特的直系同源基因座,以解决非模型系统内的浅层和深层进化关系。本文中,我们介绍了AHE方法的一种修改形式,扩大了其在蜘蛛中的应用范围,尤其着重于下层Mygalomorphae。

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