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首页> 美国卫生研究院文献>BMC Developmental Biology >A detailed staging scheme for late larval development in Strongylocentrotus purpuratus focused on readily-visible juvenile structures within the rudiment
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A detailed staging scheme for late larval development in Strongylocentrotus purpuratus focused on readily-visible juvenile structures within the rudiment

机译：一个详细的分期计划用于紫背轮虫的后期幼体发育的研究着眼于雏形内易于观察的少年结构

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BackgroundThe purple sea urchin, Strongylocentrotus purpuratus, has long been the focus of developmental and ecological studies, and its recently-sequenced genome has spawned a diversity of functional genomics approaches. S. purpuratus has an indirect developmental mode with a pluteus larva that transforms after 1–3 months in the plankton into a juvenile urchin. Compared to insects and frogs, mechanisms underlying the correspondingly dramatic metamorphosis in sea urchins remain poorly understood. In order to take advantage of modern techniques to further our understanding of juvenile morphogenesis, organ formation, metamorphosis and the evolution of the pentameral sea urchin body plan, it is critical to assess developmental progression and rate during the late larval phase. This requires a staging scheme that describes developmental landmarks that can quickly and consistently be used to identify the stage of individual living larvae, and can be tracked during the final two weeks of larval development, as the juvenile is forming.
机译：背景技术紫海胆（Strongylocentrotus purpuratus）长期以来一直是开发和生态研究的重点，其最近测序的基因组产生了多种功能基因组学方法。紫癜链球菌具有间接发育方式，其中的一个侧柏幼虫在浮游生物中经过1-3个月后会转变为幼小顽童。与昆虫和青蛙相比，海胆中相应的急剧变态的潜在机制仍然知之甚少。为了利用现代技术来进一步了解幼虫的形态发生，器官形成，变态和五旬期海胆身体计划的演变，评估幼虫后期的发育进程和速率至关重要。这就需要一个分阶段方案来描述发育标志，这些标志可以快速，一致地用于识别单个活幼体的阶段，并且可以在幼体形成的最后两周内对其进行跟踪。

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