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Routes to improving the reliability of low level DNA analysis using real-time PCR

机译：使用实时PCR提高低水平DNA分析可靠性的途径

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BackgroundAccurate quantification of DNA using quantitative real-time PCR at low levels is increasingly important for clinical, environmental and forensic applications. At low concentration levels (here referring to under 100 target copies) DNA quantification is sensitive to losses during preparation, and suffers from appreciable valid non-detection rates for sampling reasons. This paper reports studies on a real-time quantitative PCR assay targeting a region of the human SRY gene over a concentration range of 0.5 to 1000 target copies. The effects of different sample preparation and calibration methods on quantitative accuracy were investigated.

机译：背景技术对于临床，环境和法医学应用，使用定量实时PCR进行低水平的DNA准确定量越来越重要。在低浓度水平（此处指少于100个目标拷贝）下，DNA定量对制备过程中的损失敏感，并且由于采样原因而遭受相当大的有效非检测率。本文报道了针对实时SPCR基因区域的实时定量PCR分析的研究，该区域的浓度范围为0.5到1000个目标拷贝。研究了不同样品制备和校准方法对定量准确性的影响。

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期刊名称 BMC Biotechnology
作者
Stephen LR Ellison; Claire A English; Malcolm J Burns; Jacquie T Keer;
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作者单位

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年(卷),期 2006(6),-1
年度 2006
页码 33
总页数 11
原文格式 PDF
正文语种
中图分类应用微生物学;
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1. Reliability of non-invasively acquired human genomic DNA as a substrate for real-time PCR-assisted analysis of genetic polymorphisms. [J] . Neuhaus T, Geisen G, Bolt HM, Archives of Toxicology . 2004,第7期

机译：无创获取的人类基因组DNA作为实时PCR辅助分析遗传多态性的底物的可靠性。
2. Reliability of non-invasively acquired human genomic DNA as a substrate for real-time PCR-assisted analysis of genetic polymorphisms. [J] . Neuhaus T, Geisen G, Bolt HM, Archives of Toxicology . 2004,第7期

机译：非侵入性获取的人类基因组DNA作为实时PCR辅助分析遗传多态性的底物的可靠性。
3. A DNA Real-Time Quantitative PCR Method Suitable for Routine Monitoring of Low Levels of Minimal Residual Disease in Chronic Myeloid Leukemia [J] . Bartley Paul A., Latham Susan, Budgen Bradley, The Journal of molecular diagnostics: JMD . 2015,第2期

机译：一种适用于常规监测慢性髓样白血病低水平最小残留疾病的DNA实时定量PCR方法
4. Design of a PCR protocol for improving reliability of PCR in DNA computing [C] . Nakatsugawa, M., Yamamoto, Evolutionary Computation, 2002. CEC '02. Proceedings of the 2002 Congress on . 2002

机译：用于提高DNA计算中PCR可靠性的PCR方案的设计
5. Honey bee (Apis mellifera L.) PCR-RFLP DNA markers obtained and used for the analysis of genetic diversity and gene flow among New World African and European populations. [D] . Suazo-Calix, Alonso. 2000

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6. Loop-mediated isothermal amplification (LAMP) reaction as viable PCR substitute for diagnostic applications: a comparative analysis study of LAMP conventional PCR nested PCR (nPCR) and real-time PCR (qPCR) based on Entamoeba histolytica DNA derived from faecal sample [O] . Phiaw Chong Foo, A. B. Nurul Najian, Nuramin A. Muhamad, 2020

机译：环介导的等温扩增（灯）反应作为可行的PCR替代诊断应用：灯常规PCR巢式PCR（NPCR）和实时PCR（QPCR）的比较分析研究基于粪便样品的EntamoEBA组织DNA
7. Routes to improving the reliability of low level DNA analysis using real-time PCR [O] . 2006

机译：使用实时PCR提高低水平DNA分析可靠性的途径

Routes to improving the reliability of low level DNA analysis using real-time PCR

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