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首页> 美国卫生研究院文献>The Journal of Neuroscience >Retinal ganglion cells in goldfish: a qualitative classification into four morphological types and a quantitative study of the development of one of them
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Retinal ganglion cells in goldfish: a qualitative classification into four morphological types and a quantitative study of the development of one of them

机译：金鱼视网膜神经节细胞：定性分类为四种形态类型并定量研究其中一种的发育

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In this paper we describe the dendritic morphology of ganglion cells that have been retrogradely stained with HRP taken up by the cut optic nerve. This technique produces an extensive Golgi-like filling of the cells. From their appearance in the retinal whole-mount, they were classified as four types, according to the sizes of the soma and dendritic field, the thickness of the primary dendrites, and the density of the arbors. Each type was subdivided according to the level(s) of stratification of the dendrites within the inner and outer plexiform layer(s) to yield a total of 15 subtypes (four for three types, three for the other). The retina of the goldfish grows by a balloon-like expansion, and by the addition of new neurons, in annuli, at the margin. Therefore, a similar cell type may be examined at a variety of stages of development in the same retina, as well as in the retinae from fish of various ages. We have used a computer-assisted microscope to do so, quantitatively, for one large and easily identified subtype. In small fish (ca. 4 cm long), the number of dendritic branch points, the total dendritic length, and the dendritic field sizes of these cells are constant inside a central zone extending to 70–80% of the retinal radius. The magnitudes of all three numeric descriptors decrease closer to the margin. In large fish (ca. 14 cm long), the central zone extends to more than 90% of the retinal radius, and the same pattern holds. The area of the dendritic fields and the total dendritic lengths are both greater in the central zone of the large fish than in the small, but the number of branches is the same in both. This suggests that once a cell has achieved the “mature” number of dendritic branches, further growth is interstitial. A comparison of dendritic morphologies across the retina shows that the pattern of dendritic outgrowth in peripheral retina is initially directed parallel to the margin, and, later, toward the margin. This suggests that dendritic growth is impeded by the dendrites present in more central retina and proceeds preferentially where they are absent. Cells of the same age are at different distances from the optic disk in the small and large retinae. In some cases, they have quite different dendritic morphologies. This implies that dendritic development depends not only on the age and subtype of the cell, but on extrinsic factors as well.(ABSTRACT TRUNCATED AT 400 WORDS)
机译：在本文中，我们描述了神经节细胞的树突形态，这些神经节细胞已被视神经切断的HRP逆行染色。这项技术可产生大量的高尔基样细胞。从它们在视网膜整个安装座中的出现，根据体细胞和树突区域的大小，初级树突的厚度和心轴的密度，将它们分为四种类型。根据内部和外部丛状层内树突的分层水平将每种类型细分，以产生总共15种亚型（三种为四种，另一种为三种）。金鱼的视网膜通过气球状扩张以及在环的边缘增加新的神经元而生长。因此，可以在同一视网膜以及来自不同年龄鱼类的视网膜中的多个发育阶段检查相似的细胞类型。我们已经使用计算机辅助显微镜对一个大型且易于识别的亚型进行了定量分析。在小鱼（长约4厘米）中，这些细胞的树突分支点数，总树突长度和树突场大小在延伸至视网膜半径70-80％的中央区域内是恒定的。所有三个数字描述符的幅度减小得更接近裕度。在大型鱼（长约14厘米）中，中央区域延伸到视网膜半径的90％以上，并且保持相同的模式。大鱼的中央区域的树突区域面积和总树突长度均大于小鱼的中央区域，但两者的分支数相同。这表明，一旦细胞达到树突状分支的“成熟”数目，进一步的生长将进入间隙。整个视网膜上树突形态的比较显示，周围视网膜中的树突生长模式最初平行于边缘，后来指向边缘。这表明树突生长受到更多中央视网膜中存在的树突的阻碍，并优先出现在没有它们的地方。相同年龄的细胞与大和小视网膜中距视盘的距离不同。在某些情况下，它们具有完全不同的树突形态。这表明树突状发育不仅取决于细胞的年龄和亚型，还取决于外在因素。（摘要截短为400字）

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