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MAKER2: an annotation pipeline and genome-database management tool for second-generation genome projects

机译:MAKER2:用于第二代基因组计划的注释管道和基因组数据库管理工具

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摘要

BackgroundSecond-generation sequencing technologies are precipitating major shifts with regards to what kinds of genomes are being sequenced and how they are annotated. While the first generation of genome projects focused on well-studied model organisms, many of today's projects involve exotic organisms whose genomes are largely terra incognita. This complicates their annotation, because unlike first-generation projects, there are no pre-existing 'gold-standard' gene-models with which to train gene-finders. Improvements in genome assembly and the wide availability of mRNA-seq data are also creating opportunities to update and re-annotate previously published genome annotations. Today's genome projects are thus in need of new genome annotation tools that can meet the challenges and opportunities presented by second-generation sequencing technologies.
机译:背景技术第二代测序技术正在促使人们对哪些类型的基因组进行测序以及如何对其进行注释进行重大改变。虽然第一代基因组计划的重点是经过充分研究的模型生物,但今天的许多计划都涉及外来生物,其外源基因组主要是兵马俑。这使它们的注释复杂化,因为与第一代项目不同,没有用于训练基因发现者的预先存在的“金标准”基因模型。基因组装配的改进和mRNA序列数据的广泛可用性也为更新和重新注释以前发布的基因组注释创造了机会。因此,当今的基因组项目需要新的基因组注释工具,以应对第二代测序技术带来的挑战和机遇。

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