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Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder

机译:在双螺杆挤出机中预处理后蒸​​汽爆破的玉米芯残留物的酶促水解

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摘要

A modified twin-screw extruder incorporated with a filtration device was used as a liquid/solid separator for xylose removal from steam exploded corncobs. A face centered central composite design was used to study the combined effects of various enzymatic hydrolysis process variables (enzyme loading, surfactant addition, and hydrolysis time) with two differently extruded corncobs (7% xylose removal, 80% xylose removal) on glucose conversion. The results showed that the extrusion process led to an increase in cellulose crystallinity, while structural changes could also be observed via SEM. A quadratic polynomial model was developed for predicting the glucose conversion and the fitted model provided an adequate approximation of the true response as verified by the analysis of variance (ANOVA).
机译:装有过滤装置的改进型双螺杆挤出机用作液/固分离器,用于从蒸汽炸玉米芯中去除木糖。以面心为中心的中央复合材料设计用于研究各种酶促水解工艺变量(酶负载,表面活性剂添加和水解时间)与两种不同挤压的玉米芯(7%木糖去除,80%木糖去除)的综合影响。结果表明,挤出过程导致纤维素结晶度的增加,同时也可以通过SEM观察到结构变化。开发了用于预测葡萄糖转化的二次多项式模型,拟合模型提供了真实响应的足够近似值,通过方差分析(ANOVA)进行了验证。

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