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Evaluation of dairy processing wastewater biotreatment in an IASBR system: Aeration rate impacts on performance and microbial ecology

机译:在IASBR系统中评估乳制品加工废水的生物处理:曝气速率对生产性能和微生物生态的影响

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摘要

Dairy processing generates large volumes of wastewater that require extensive nutrient remediation prior to discharge. Significant commercial opportunities exist therefore for cost-effective biotechnologies capable of achieving this requirement. In this study the authors evaluated the use of intermittently aerated sequencing batch reactors, (IASBRs), as a single-tank biotreatment system for co-removal of COD, nitrogen and phosphorus from synthetic dairy processing wastewater. Variation of the IASBR aeration rates, (0.8, 0.6 and 0.4 L/min), had significant impacts on the respective nutrient removal efficiencies and underlying microbial diversity profiles. Aeration at 0.6 L/min was most effective and resulted in >90% co-removal of orthophosphate and ammonium. 16S rRNA based pyrosequencing of biomass DNA samples revealed the family Comamonadaceae was notably enriched (>80% relative abundance) under these conditions. In silico predictive metabolic modelling also identified Comamonadaceae as the major contributor of several known genes for nitrogen and phosphorus assimilation (nirK, nosZ, norB, ppK, ppX and phbC).
机译:乳制品加工会产生大量废水,在排放之前,这些废水需要大量的养分修复。因此,对于能够实现这一要求的具有成本效益的生物技术而言,存在巨大的商业机会。在这项研究中,作者评估了间歇充气顺序分批反应器(IASBR)作为单罐生物处理系统的用途,以从合成乳制品加工废水中共同去除COD,氮和磷。 IASBR曝气速率(0.8、0.6和0.4?L / min)的变化对各自的养分去除效率和潜在的微生物多样性特征有重大影响。以0.6?L / min的曝气效率最高,可将正磷酸盐和铵的共去除率> 90%。基于16S rRNA的生物质DNA样品焦磷酸测序表明,在这些条件下,Comamonadaceae家族显着富集(相对丰度> 80%)。在计算机上,预测代谢建模还确定了昏迷科是氮和磷同化的几种已知基因(nirK,nosZ,norB,ppK,ppX和phbC)的主要贡献者。

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