• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>BioMed Research International >A Novel Lectin with Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Dried Fruiting Bodies of the Monkey Head Mushroom Hericium erinaceum
【2h】

A Novel Lectin with Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Dried Fruiting Bodies of the Monkey Head Mushroom Hericium erinaceum

机译：新型的具有抗增殖和HIV-1逆转录酶抑制活性的猴头菇猴头菇干果体凝集素。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A lectin designated as Hericium erinaceum agglutinin (HEA) was isolated from dried fruiting bodies of the mushroom Hericium erinaceum with a chromatographic procedure which entailed DEAE-cellulose, CM-cellulose, Q-Sepharose, and FPLC Superdex 75. Its molecular mass was estimated to be 51 kDa and its N-terminal amino acid sequences was distinctly different from those of other isolated mushroom lectins. The hemagglutinating activity of HEA was inhibited at the minimum concentration of 12.5 mM by inulin. The lectin was stable at pH 1.9–12.1 and at temperatures up to 70°C, but was inhibited by Hg2+, Cu2+, and Fe3+ ions. The lectin exhibited potent mitogenic activity toward mouse splenocytes, and demonstrated antiproliferative activity toward hepatoma (HepG2) and breast cancer (MCF7) cells with an IC50 of 56.1 μM and 76.5 μM, respectively. It manifested HIV-1 reverse transcriptase inhibitory activity with an IC50 of 31.7 μM. The lectin exhibited potent mitogenic activity toward murine splenocytes but was devoid of antifungal activity.
机译：用色谱方法从蘑菇猴头菇的干燥子实体中分离出一种被称为猴头菇凝集素（HEA）的凝集素，该方法需要DEAE-纤维素，CM-纤维素，Q-琼脂糖和FPLC Superdex75。其分子量估计为其分子量为51 kDa，其N端氨基酸序列与其他分离的蘑菇凝集素明显不同。菊粉在最低浓度为12.5 mM时抑制了HEA的血凝活性。凝集素在pH 1.9–12.1和高达70°C的温度下均稳定，但受到Hg 2 + ，Cu 2 + 和Fe 3的抑制+ 离子。凝集素对小鼠脾细胞表现出有力的促有丝分裂活性，对肝细胞（HepG2）和乳腺癌（MCF7）细胞具有抗增殖活性，IC50分别为56.1μM和76.5μM。它表现出HIV-1逆转录酶抑制活性，IC50为31.7μM。凝集素对鼠脾细胞表现出有力的促有丝分裂活性，但没有抗真菌活性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号