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Fabrication of Biocompatible Vibrational Magnetoelastic Materials for Controlling Cellular Adhesion

机译:用于控制细胞粘附的生物相容性振动磁弹性材料的制备

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摘要

This paper describes the functionalization of magnetoelastic (ME) materials with Parylene-C coating to improve the surface reactivity to cellular response. Previous study has demonstrated that vibrating ME materials were capable of modulating cellular adhesion when activated by an externally applied AC magnetic field. However, since ME materials are not inherently biocompatible, surface modifications are needed for their implementation in biological settings. Here, the long-term stability of the ME material in an aqueous and biological environment is achieved by chemical-vapor deposition of a conformal Parylene-C layer, and further functionalized by methods of oxygen plasma etching and protein adsorption. In vitro cytotoxicity measurement and characterization of the vibrational behavior of the ME materials showed that Parylene-C coatings of 10 µm or greater could prevent hydrolytic degradation without sacrificing the vibrational behavior of the ME material. This work allows for long-term durability and functionality of ME materials in an aqueous and biological environment and makes the potential use of this technology in monitoring and modulating cellular behavior at the surface of implantable devices feasible.
机译:本文介绍了具有Parylene-C涂层的磁弹性(ME)材料的功能化,以改善对细胞反应的表面反应性。先前的研究表明,振动的ME材料在被外部施加的AC磁场激活时能够调节细胞粘附。但是,由于ME材料本身不是生物相容的,因此需要对其进行表面修饰以在生物环境中实现。在此,ME材料在水性和生物环境中的长期稳定性是通过化学气相沉积保形的Parylene-C层实现的,并通过氧等离子体蚀刻和蛋白质吸附的方法进一步实现功能化。体外细胞毒性测量和ME材料的振动行为表征表明,10μm或更大的Parylene-C涂层可以在不牺牲ME材料的振动行为的情况下防止水解降解。这项工作允许ME材料在水性和生物环境中具有长期耐久性和功能性,并使该技术在监视和调节可植入设备表面的细胞行为方面的潜在应用成为可能。

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