• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Biophysical Journal >Myoplasmic binding of fura-2 investigated by steady-state fluorescence and absorbance measurements.
【2h】

Myoplasmic binding of fura-2 investigated by steady-state fluorescence and absorbance measurements.

机译：通过稳态荧光和吸光度测量研究了呋喃2的胞质结合。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Binding of the fluorescent Ca2+ indicator dye fura-2 by intracellular constituents has been investigated by steady-state optical measurements. Fura-2's (a) fluorescence intensity, (b) fluorescence emission anisotropy, (c) fluorescence emission spectrum, and (d) absorbance spectra were measured in glass capillary tubes containing solutions of purified myoplasmic proteins; properties b and c were also measured in frog skeletal muscle fibers microinjected with fura-2. The results indicate that more than half, and possibly as much as 85%, of fura-2 molecules in myoplasm are in a protein-bound form, and that the binding changes many properties of the dye. For example, in vitro characterization of the Ca2+-dye reaction indicates that when fura-2 is bound to aldolase (a large and abundant myoplasmic protein), the dissociation constant of the dye for Ca2+ is three- to fourfold larger than that measured in the absence of protein. The problems raised by intracellular binding of fura-2 to cytoplasmic proteins may well apply to cells other than skeletal muscle fibers.
机译：荧光Ca2 +指示剂fura-2与细胞内成分的结合已通过稳态光学测量进行了研究。在装有纯化的肌浆蛋白溶液的玻璃毛细管中测量Fura-2的（a）荧光强度，（b）荧光发射各向异性，（c）荧光发射光谱和（d）吸收光谱。还通过显微注射呋喃2来测量青蛙的骨骼肌纤维的b和c特性。结果表明，肌浆中呋喃2分子的一半以上（可能多达85％）处于蛋白结合形式，并且这种结合改变了染料的许多特性。例如，Ca2 +-染料反应的体外表征表明，当fura-2与醛缩酶（大量大量的肌浆蛋白）结合时，该染料对Ca2 +的解离常数比在C3 +中测得的解离常数大三到四倍。没有蛋白质。呋喃2与胞质蛋白的胞内结合引起的问题很可能适用于除骨骼肌纤维以外的细胞。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号