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首页> 美国卫生研究院文献>Biophysical Journal >A neutron reflectivity study of polymer-modified phospholipid monolayers at the solid-solution interface: polyethylene glycol-lipids on silane-modified substrates.
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A neutron reflectivity study of polymer-modified phospholipid monolayers at the solid-solution interface: polyethylene glycol-lipids on silane-modified substrates.

机译：固溶体界面上聚合物修饰的磷脂单层的中子反射率研究：硅烷修饰基质上的聚乙二醇-脂质。

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摘要

The structure of polymer-decorated phospholipid monolayers at the solid-solution interface was investigated using neutron reflectometry. The monolayers were composed of distearoylphosphatidylethanolamine (DSPE) matrixed with varying amounts of DSPE-PEG (DSPE with polyethylene glycol covalently grafted to its headgroup). Mixed lipid monolayers were Langmuir-Blodgett deposited onto hydrophobic quartz or silicon substrates, previously hydrophobized by chemically grafting a robust monolayer of octadecyltrichlorosilane (OTS). We show that this method results in homogeneous and continuous phospholipid monolayers on the silanated substrates and determine that the grafted PEG chains extend away from the monolayers into the solvent phase as a function of their density, as expected from scaling theories. In addition, ligands were coupled to the end of the PEG chains and selective binding was demonstrated using fluorescence microscopy. Our results demonstrate that these constructs are ideal for further characterization and studies with well-defined monomolecular films.
机译：使用中子反射法研究了固溶界面上聚合物修饰的磷脂单层的结构。单层由二硬脂酰磷脂酰乙醇胺（DSPE）和不同量的DSPE-PEG（DSPE的聚乙二醇共价接枝到其头基上）组成。混合脂质单层被Langmuir-Blodgett沉积在疏水性石英或硅基板上，事先通过化学接枝坚固的十八烷基三氯硅烷（OTS）单层进行了疏水化处理。我们表明，该方法导致硅烷化底物上的均质且连续的磷脂单层，并确定了接枝的PEG链作为密度的函数从单层延伸到溶剂相，这是缩放理论所期望的。另外，将配体偶联至PEG链的末端，并使用荧光显微镜证实了选择性结合。我们的结果表明，这些构建体非常适合于使用明确的单分子膜进行进一步的表征和研究。

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