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首页> 美国卫生研究院文献>Biophysical Journal >Real-Time Monitoring of Platelet Activation Using Quartz Thickness-Shear Mode Resonator Sensors
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Real-Time Monitoring of Platelet Activation Using Quartz Thickness-Shear Mode Resonator Sensors

机译：使用石英厚度-剪切模式谐振器传感器实时监测血小板活化

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In this study, quartz thickness-shear mode (TSM) resonator sensors were adopted to monitor the process of platelet activation. Resting platelets adhering to fibrinogen-coated electrodes were activated by different concentrations of thrombin (1, 10, and 100 U/mL), and the corresponding electrical admittance spectra of TSM resonators during this process were recorded. Based on a bilayer-loading transmission line model of TSM resonators, the complex shear modulus (G′ + jG″) and the average thickness (hPL) of the platelet monolayer at a series of time points were obtained. Decrease in thrombin concentration from 100 to 1 U/mL shifted all peaks and plateaus in G′, G″, and hPL to higher time points, which could be attributed to the partial activation of platelets by low concentrations of thrombin. The peak value of hPL was acquired when platelets presented their typical spherical shape as the first transformation in activation process. The G′ peak appeared 10 ∼ 20 min after hPL peak, when some filopods were observed along the periphery of platelets but without obvious cell spreading. As platelet spreading began and continued, G′, G″, and hPL decreased, leading to a steady rise of resonance frequency shift of TSM resonator sensors. The results show high reliability and stability of TSM resonator sensors in monitoring the process of platelet activation, revealing an effective method to measure platelet activities in real-time under multiple experimental conditions. The G′, G″, and hPL values could provide useful quantitative measures on platelet structure variations in activation process, indicating potential of TSM resonators in characterization of cells during their transformation.
机译：在这项研究中，采用石英厚度剪切模式（TSM）谐振器传感器来监测血小板活化过程。粘附在纤维蛋白原涂层电极上的静息血小板被不同浓度的凝血酶（1、10和100U / mL）激活，并记录了该过程中TSM谐振器的相应电导率谱。基于TSM谐振器的双层加载传输线模型，在一系列时间点获得了血小板单层的复数剪切模量（G'+ jG''）和平均厚度（hPL）。凝血酶浓度从100U / mL降低到G'，G''和hPL中的所有峰和平台都移到了更高的时间点，这可能归因于低浓度的凝血酶对血小板的部分活化。当血小板呈现其典型的球形形状作为活化过程中的第一个转化时，可获得hPL的峰值。 GPL峰在hPL峰之后10到20分钟出现，当时在血小板外围观察到一些丝虫，但没有明显的细胞扩散。随着血小板扩散的开始和持续，G'，G''和h PL 减小，导致TSM谐振器传感器的谐振频率漂移稳定上升。结果表明，TSM谐振器传感器在监测血小板活化过程中具有很高的可靠性和稳定性，这揭示了一种在多种实验条件下实时测量血小板活性的有效方法。 G '， G ''和 h PL 值可以为激活血小板结构变化提供有用的定量方法这个过程表明了TSM谐振器在转换过程中表征电池的潜力。

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