A rapid and simple technique is proposed for methanol concentration detection using a PMMA (Polymethyl-Methacrylate) microfluidic chip patterned using a commercially available CO2 laser scriber. In the proposed device, methanol and methanol oxidase (MOX) are injected into a three-dimensional circular chamber and are mixed via a vortex stirring effect. The mixture is heated to prompt the formation of formaldehyde and is flowed into a rectangular chamber, to which fuchsin-sulphurous acid is then added. Finally, the microchip is transferred to a UV spectrophotometer for methanol detection purposes. The experimental results show that a correlation coefficient of R2 = 0.9940 is obtained when plotting the optical density against the methanol concentration for samples and an accuracy as high as 93.1% are compared with the determined by the high quality gas chromatography with concentrations in the range of 2 ∼ 100 ppm. The methanol concentrations of four commercial red wines are successfully detected using the developed device. Overall, the results show that the proposed device provides a rapid and accurate means of detecting the methanol concentration for a variety of applications in the alcoholic beverage inspection and control field.
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机译：提出了一种快速而简单的技术，用于使用PMMA（聚甲基丙烯酸甲酯）微流控芯片对甲醇浓度进行检测，该芯片采用可商购的CO2激光划片机进行图案化。在提出的装置中，将甲醇和甲醇氧化酶（MOX）注入三维圆形腔室中，并通过涡流搅拌作用进行混合。加热混合物以促使甲醛形成，并流入矩形腔室，然后向其中加入品红-亚硫酸。最后，将微芯片转移到紫外分光光度计中进行甲醇检测。实验结果表明，将光密度对甲醇浓度作图可得到R 2 up = 0.9940的相关系数，并且与由高品质测定的样品相比，其准确度高达93.1％。气相色谱法，浓度范围为2〜100 ppm。使用开发的设备可以成功检测出四种商用红酒的甲醇浓度。总体而言，结果表明，所提出的装置为酒精饮料检查和控制领域中的各种应用提供了一种快速准确的检测甲醇浓度的方法。
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