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Inverting microwell array chip for the cultivation of human induced pluripotent stem cells with controlled aggregate size and geometrical arrangement

机译：倒置微孔阵列芯片用于培养具有受控聚集体大小和几何排列的人诱导多能干细胞

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摘要

We present a novel cell culture chip, namely, “inverting microwell array chip,” for cultivation of human induced pluripotent stem cells. The chip comprises a lower hydrogel microwell array and an upper polystyrene culture surface. We demonstrate the formation of uniform cellular aggregates in the microwell array, and after inversion, a culture with controlled aggregate size and geometrical arrangement on the polystyrene surface. Here, we report effects of cell concentrations on a cultivation sequence in the chip.

机译：我们提出了一种新型的细胞培养芯片，即“反相微孔阵列芯片”，用于培养人诱导的多能干细胞。该芯片包括下部水凝胶微孔阵列和上部聚苯乙烯培养表面。我们证明了在微孔阵列中形成均匀的细胞聚集体，并且在转化后，在聚苯乙烯表面上具有可控制的聚集体尺寸和几何排列的培养。在这里，我们报告细胞浓度对芯片中培养序列的影响。

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期刊名称 Biomicrofluidics
作者
Taku Satoh; Shinji Sugiura; Kimio Sumaru; Shigenori Ozaki; Shinichi Gomi; Tomoaki Kurakazu; Yasuhiro Oshima; Toshiyuki Kanamori;
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作者单位

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年(卷),期 2014(8),2
年度 2014
页码 024112
总页数 7
原文格式 PDF
正文语种
中图分类生物物理学;
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专利

1. Formation of Well-defined Embryoid Bodies from Dissociated Human Induced Pluripotent Stem Cells using Microfabricated Cell-repellent Microwell Arrays [J] . Giuseppe Pettinato, Xuejun Wen, Ning Zhang Scientific reports. . 2014,第1期

机译：使用微细的细胞排斥微孔阵列，从解离的人类诱导多能干细胞中形成明确的胚状体。
2. Developing a Cost-Effective and Scalable Production of Human Hepatic Competent Endoderm from Size-Controlled Pluripotent Stem Cell Aggregates [J] . Farzaneh Zahra, Najarasl Mostafa, Abbasalizadeh Saeed, Stem cells and development . 2018,第4期

机译：从尺寸控制的多能干细胞聚集体中制定成本效益和可扩展的人类肝脏态态内胚层的生产
3. Bioprocess development for mass production of size-controlled human pluripotent stem cell aggregates in stirred suspension bioreactor [J] . AbbasalizadehS., LarijaniM.R., SamadianA., Tissue engineering, Part C. Methods . 2012,第11期

机译：在搅拌悬浮生物反应器中大规模生产尺寸受控的人多能干细胞聚集体的生物工艺开发
4. BIOPROCESS INTENSIFICATION FOR THE CONTINUOUS EXPANSION OF 3D HUMAN INDUCED PLURIPOTENT STEM CELL AGGREGATES IN BIOREACTORS [C] . Bernardo Abecasis, Tiago Aguiar, Emilie Arnault, Conference on single-use technologies II: bridging polymer science to biotechnology applications . 2017

机译：生物过程强化对3D人类诱导的多潜能干细胞在生物反应器中聚集的持续扩展
5. Geometric Control of Cardiomyogenic Induction from Human Pluripotent Stem Cells. [D] . Bauwens, Celine Liu. 2010

机译：人多能干细胞引起心肌病的几何控制。
6. Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells [O] . Celine L. Bauwens, Derek Toms, Mark Ungrin 2016

机译：悬浮细胞的人多能干细胞心脏分化的微孔中的总大小优化。
7. Formation of Well-defined Embryoid Bodies from Dissociated Human Induced Pluripotent Stem Cells using Microfabricated Cell-repellent Microwell Arrays [O] . Giuseppe Pettinato, Xuejun Wen, Ning Zhang 2014

机译：使用微羧细胞排斥微孔阵列形成来自解离人诱导的多能干细胞的明确定义的胚状体

Inverting microwell array chip for the cultivation of human induced pluripotent stem cells with controlled aggregate size and geometrical arrangement

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