• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Biomicrofluidics >Evaporation-induced monolayer compression improves droplet interface bilayerformation using unsaturated lipids
【2h】

Evaporation-induced monolayer compression improves droplet interface bilayerformation using unsaturated lipids

机译：蒸发诱导的单层压缩可改善液滴界面双层使用不饱和脂质形成

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this article, we report on a new experimental methodology to enable reliable formation of droplet interface bilayer (DIB) model membranes with two types of unsaturated lipids that have proven difficult for creating stable DIBs. Through the implementation of a simple evaporation technique to condition the spontaneously assembled lipid monolayer around each droplet, we increased the success rates of DIB formation for two distinct unsaturated lipids, namely 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), from less than 10% to near 100%. Separately, using a pendant drop tensiometer, we learned that: (a) DOPC and POPC monolayers do not spontaneously assemble into their tightest possible configurations at an oil-water interface, and (b) reducing the surface area of a water droplet coated with a partially packed monolayer leads to a more tightly packed monolayer with an interfacial tension lower than that achieved by spontaneous assembly alone. We also estimated from Langmuir compression isotherms obtained for both lipids that the brief droplet evaporation procedure prior to DIB formation resulted in a 6%–16% reduction in area per lipid for DOPC and POPC, respectively. Finally, the increased success rates of formation for DOPC and POPC DIBs enabled quantitative characterization of unsaturated lipid membrane properties including electrical resistance, rupture potential, and specificcapacitance.
机译：在本文中，我们报告了一种新的实验方法，该方法能够可靠地形成具有两种类型的不饱和脂质的液滴界面双层（DIB）模型膜，这些膜已被证明很难产生稳定的DIB。通过实施简单的蒸发技术来调节每个液滴周围自发组装的脂质单层，我们提高了两种不同的不饱和脂质即1,2-二油酰基-sn-甘油-3-磷酸胆碱（DOPC）的DIB形成成功率。和1-棕榈酰-2-油酰基-sn-甘油-3-磷酸胆碱（POPC），从少于10％降至接近100％。另外，我们使用悬垂式悬吊式张力计，了解到：（a）DOPC和POPC单层不会在油水界面上自发地组装成最紧密的配置，并且（b）减小涂有硅藻土的水滴的表面积部分填充的单分子层会导致紧密结合的单分子层，其界面张力要比仅通过自发组装获得的界面张力低。我们还从Langmuir对两种脂质获得的压缩等温线中估计，DIB形成之前的短暂液滴蒸发程序分别使DOPC和POPC的脂质面积减少了6％–16％。最后，DOPC和POPC DIB形成成功率的提高使得能够定量表征不饱和脂质膜的特性，包括电阻，断裂电位和比电容。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号