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首页> 美国卫生研究院文献>Biomedical Optics Express >Rhodamine B as an optical thermometer in cells focally exposed to infrared laser light or nanosecond pulsed electric fields
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Rhodamine B as an optical thermometer in cells focally exposed to infrared laser light or nanosecond pulsed electric fields

机译：罗丹明B作为光学温度计在细胞中集中暴露于红外激光或纳秒脉冲电场

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The temperature-dependent fluorescence property of Rhodamine B was used to measure changes in temperature at the cellular level induced by either infrared laser light exposure or high intensity, ultrashort pulsed electric fields. The thermal impact of these stimuli were demonstrated at the cellular level in time and contrasted with the change in temperature observed in the extracellular bath. The method takes advantage of the temperature sensitivity of the fluorescent dye Rhodamine B which has a quantum yield linearly dependent on temperature. The thermal effects of different temporal pulse applications of infrared laser light exposure and of nanosecond pulsed electric fields were investigated. The temperature increase due to the application of nanosecond pulsed electric fields was demonstrated at the cellular level.
机译：罗丹明B的随温度变化的荧光特性被用于测量红外激光照射或高强度超短脉冲电场引起的细胞水平温度变化。这些刺激的热影响在细胞水平上得到及时证明，并与在细胞外浴中观察到的温度变化形成对比。该方法利用了荧光染料若丹明B的温度敏感性，该染料的量子产率与温度成线性关系。研究了不同时间脉冲施加红外激光和纳秒脉冲电场的热效应。在细胞水平上证实了由于施加纳秒脉冲电场而引起的温度升高。

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