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Transient absorption imaging of hemes with 2-color independently tunable visible-wavelength ultrafast source

机译:利用2色独立可调的可见光超快光源对血红素进行瞬态吸收成像

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摘要

Pump probe microscopy is a time-resolved multiphoton imaging technique capable of generating contrast between non-fluorescent pigments based on differences in excited-state lifetimes. Here we describe a fiber-based ultrafast system designed for imaging heme proteins with an independently-tunable pulse pair in the visible-wavelength regime. Starting with a 1060 nm fiber amplifier (1.3 W at 63 MHz, 140 fs pulses), visible pulses were produced in the vicinity of 488 nm and 532 nm by doubling the output of a short photonic crystal fiber with a pair of periodically-poled lithium niobate crystals, providing 5–20 mW power in each beam. This was sufficient for acquiring transient absorption images from unstained cryosectioned tissue.
机译:泵浦探针显微镜是一种时间分辨的多光子成像技术,能够根据激发态寿命的差异在非荧光颜料之间产生对比度。在这里,我们描述了一种基于纤维的超快系统,该系统设计用于在可见波长范围内使用独立可调的脉冲对成像血红素蛋白。从1060 nm光纤放大器(63 MHz时为1.3 W,140 fs脉冲)开始,通过将短光子晶体光纤的输出与一对周期性极化的锂成倍增加,在488 nm和532 nm附近产生可见脉冲。铌酸盐晶体,每个光束提供5–20 mW的功率。这足以从未染色的冷冻切片组织中获取瞬态吸收图像。

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