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Label-free detection of hydrogen peroxide-induced oxidative stress in human retinal pigment epithelium cells via laser tweezers Raman spectroscopy

机译:激光镊子拉曼光谱法无标记检测人视网膜色素上皮细胞中过氧化氢诱导的氧化应激

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摘要

Human retinal pigment epithelium cells under hydrogen peroxide-induced oxidative stress and a ligustrazine-based protective effect were investigated using laser tweezers Raman spectroscopy. Protein and lipid were significantly affected by oxidative damage, along with increased reactive oxygen species (ROS) level within cells. The effects of ligustrazine against the reaction of ROS with protein seemed to be able to inhibit such damages but were limited during the desamidization of amides, along with additional effect on nucleic acid base and DNA phosphoric acid skeleton. This work laid the basis for both understanding the molecular mechanisms of oxidative stress-induced injury and highlighting possible biomarkers in retinal diseases.
机译:使用激光镊子拉曼光谱研究过氧化氢诱导的氧化应激和基于川gust嗪的人视网膜色素上皮细胞。蛋白质和脂质受氧化损伤的影响很大,同时细胞内的活性氧(ROS)水平也升高。川gust嗪对ROS与蛋白质反应的影响似乎能够抑制这种破坏,但在酰胺脱酰胺化过程中受到限制,并且对核酸碱基和DNA磷酸骨架具有附加作用。这项工作为了解氧化应激诱导的损伤的分子机制以及强调视网膜疾病中可能的生物标志物奠定了基础。

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