• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Journal of Pharmaceutical Analysis >Accurate quantitation standards of glutathione via traceable sulfur measurement by inductively coupled plasma optical emission spectrometry and ion chromatography
【2h】

Accurate quantitation standards of glutathione via traceable sulfur measurement by inductively coupled plasma optical emission spectrometry and ion chromatography

机译：通过电感耦合等离子体发射光谱法和离子色谱法可追溯的硫测量来测定谷胱甘肽的准确定量标准

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The quantitative analysis of glutathione (GSH) is important in different fields like medicine, biology, and biotechnology. Accurate quantitative measurements of this analyte have been hampered by the lack of well characterized reference standards. The proposed procedure is intended to provide an accurate and definitive method for the quantitation of GSH for reference measurements. Measurement of the stoichiometrically existing sulfur content in purified GSH offers an approach for its quantitation and calibration through an appropriate characterized reference material (CRM) for sulfur would provide a methodology for the certification of GSH quantity, that is traceable to SI (International system of units). The inductively coupled plasma optical emission spectrometry (ICP-OES) approach negates the need for any sample digestion. The sulfur content of the purified GSH is quantitatively converted into sulfate ions by microwave-assisted UV digestion in the presence of hydrogen peroxide prior to ion chromatography (IC) measurements. The measurement of sulfur by ICP-OES and IC (as sulfate) using the “high performance” methodology could be useful for characterizing primary calibration standards and certified reference materials with low uncertainties. The relative expanded uncertainties (% U) expressed at 95% confidence interval for ICP-OES analyses varied from 0.1% to 0.3%, while in the case of IC, they were between 0.2% and 1.2%. The described methods are more suitable for characterizing primary calibration standards and certifying reference materials of GSH, than for routine measurements.
机译：谷胱甘肽（GSH）的定量分析在医学，生物学和生物技术等不同领域都非常重要。由于缺乏良好表征的参考标准，该分析物的准确定量测量受到阻碍。拟议的程序旨在为参考测量的GSH定量提供一种准确而确定的方法。纯化的GSH中化学计量存在的硫含量的测量提供了一种通过适当的硫特征性参考物质（CRM）进行定量和校准的方法，这将提供GSH量认证的方法，该方法可追溯至SI（国际单位制） ）。电感耦合等离子体发射光谱法（ICP-OES）消除了对任何样品消解的需要。在离子色谱（IC）测量之前，在过氧化氢的存在下，通过微波辅助的UV消化，将纯化的GSH的硫含量定量转化为硫酸根离子。使用“高性能”方法通过ICP-OES和IC（作为硫酸盐）进行硫的测量可能有助于表征主要的校准标准品和具有较低不确定性的认证参考材料。 ICP-OES分析以95％置信区间表示的相对扩展不确定度（％U）在0.1％至0.3％之间，而对于IC，则在0.2％至1.2％之间。所描述的方法比常规测量更适合于表征主要校准标准和鉴定GSH的参考物质。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号