首页> 美国卫生研究院文献>Biodiversity Data Journal >Spider hosts (Arachnida Araneae) and wasp parasitoids (Insecta Hymenoptera Ichneumonidae Ephialtini) matched using DNA barcodes
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Spider hosts (Arachnida Araneae) and wasp parasitoids (Insecta Hymenoptera Ichneumonidae Ephialtini) matched using DNA barcodes

机译:使用DNA条码匹配的蜘蛛宿主(蛛形纲蛛形纲)和黄蜂寄生的拟寄生物(昆虫纲膜翅目I科E纲)

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摘要

The study of parasitoids and their hosts suffers from a lack of reliable taxonomic data. We use a combination of morphological characters and DNA sequences to produce taxonomic determinations that can be verified with reference to specimens in an accessible collection and DNA barcode sequences posted to the Barcode of Life database (BOLD). We demonstrate that DNA can be successfully extracted from consumed host spiders and the shed pupal case of a wasp using non-destructive methods. We found Acrodactyla quadrisculpta to be a parasitoid of Tetragnatha montana; Zatypota percontatoria and Zatypota bohemani both are parasitoids of Neottiura bimaculata. Zatypota anomala is a parasitoid of an as yet unidentified host in the family Dictynidae, but the host species may be possible to identify in the future as the library of reference sequences on BOLD continues to grow. The study of parasitoids and their hosts traditionally requires specialized knowledge and techniques, and accumulating data is a slow process. DNA barcoding could allow more professional and amateur naturalists to contribute data to this field of study. A publication venue dedicated to aggregating datasets of all sizes online is well suited to this model of distributed science.
机译:寄生物及其宿主的研究因缺乏可靠的分类学数据而受苦。我们使用形态特征和DNA序列的组合来产生分类学测定,可以参考可访问的集合中的标本和发布到生命条形码数据库(BOLD)中的DNA条形码序列进行验证。我们证明,可以使用非破坏性方法成功地从消耗的宿主蜘蛛和黄蜂的脱落p病例中成功提取DNA。我们发现Acrodactyla quadrisculpta是Tetragnatha montana的寄生虫。 Zatypota percontatoria和Zatypota bohemani都是 Neottiura bimaculata 的寄生虫。 em> Zatypota anomala 是寄生虫 Dictynidae 家族中尚未被鉴定的寄主,但将来有可能鉴定为BOLD上的参考序列库继续增长。传统上,对寄生虫及其宿主的研究需要专门的知识和技术,并且积累数据是一个缓慢的过程。 DNA条码可以使更多的专业和业余博物学家为该研究领域贡献数据。专门用于在线汇总各种规模数据集的发布场所非常适合这种分布式科学模型。

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