• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Biochemical Journal >Purification and properties of a soluble reduced nicotinamide–adenine dinucleotide (phosphate) dehydrogenase from the hepatopancreas of Octopus vulgaris
【2h】

Purification and properties of a soluble reduced nicotinamide–adenine dinucleotide (phosphate) dehydrogenase from the hepatopancreas of Octopus vulgaris

机译：八达通肝胰脏中可溶性还原烟酰胺-腺嘌呤二核苷酸（磷酸）脱氢酶的纯化和性质

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

1. The oxidation of NADH and NADPH catalysed by the soluble supernatant from the hepatopancreas of Octopus vulgaris is due to a single enzyme, which has been purified approximately 100-fold. The enzyme reacts rapidly with potassium ferricyanide, and more slowly with 2,6-dichlorophenol-indophenol. No activity is obtained with oxygen, cytochrome c, lipoic acid, vitamin K1, vitamin K3, ubiquinone-30, p-benzoquinone, 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium chloride or methylene blue. 2. GSH, cysteine and mercaptoethanol stimulate the enzymic activity up to fivefold. GSSG is without any apparent effect. When stimulated by GSH the enzyme becomes sensitive to dicoumarol, which produces an inhibition competitive with respect to the activator. 3. The purified enzyme contains an acid-removable flavine component, which has been identified as FMN by spectrofluorimetry and chromatography in three solvent systems. After acid ammonium sulphate treatment the enzymic activity is lost, but it can be almost fully restored by incubation with FMN. FAD produces only a partial reactivation.
机译：1.来自普通章鱼肝胰脏的可溶性上清液催化的NADH和NADPH的氧化归因于单一酶，该酶已纯化约100倍。该酶与铁氰化钾反应迅速，而与2,6-二氯苯酚-吲哚酚反应较慢。用氧气，细胞色素c，硫辛酸，维生素K1，维生素K3，泛醌-30，对苯醌，2-对碘苯基-3-对硝基苯基-5-苯基四唑氯化物或亚甲基蓝没有活性。 2. GSH，半胱氨酸和巯基乙醇最多可激发五倍的酶活性。 GSSG没有任何明显的作用。当受到GSH刺激时，该酶对双香豆酚敏感，从而产生相对于活化剂竞争的抑制作用。 3.纯化的酶含有一种可酸去除的黄素成分，通过分光荧光法和色谱法在三种溶剂系统中鉴定为FMN。酸性硫酸铵处理后，酶活性丧失，但通过与FMN孵育几乎可以完全恢复。 FAD仅产生部分重新激活。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号