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Properties and substrate specificity of the leucyl- the threonyl- and the valyl-transfer-ribonucleic acid synthetases from Aesculus species

机译：七叶树属物种的亮氨酰基-苏氨酰基-和戊基转移-核糖核酸合成酶的性质和底物特异性

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1. Leucyl- and threonyl-tRNA synthetases were partially purified up to 100-fold and 30-fold respectively from cotyledons of Aesculus hippocastanum and were largely separated from the other aminoacyl-tRNA synthetases. Valyl-tRNA synthetase was purified 25-fold from cotyledons of Aesculus californica. 2. Some properties are reported for the three enzymes when assayed by the [³²P]pyrophosphate-ATP exchange technique. 3. β-(Methylenecyclopropyl)alanine, isoleucine, azaleucine, norleucine and γ-hydroxynorvaline acted as alternative substrates for the leucyl-tRNA synthetase; the enzyme's affinity for β-(methylenecyclopropyl)-alanine and for isoleucine was about 80-fold less than that exhibited for leucine. 4. α-Cyclopropylglycine and α-cyclobutylglycine acted as alternative substrates for the valyl-tRNA synthetase.

机译：1.从欧洲七叶树的子叶分别纯化部分亮氨酸和苏氨酰-tRNA合成酶分别至多100倍和30倍，并与其他氨酰基-tRNA合成酶基本分开。从加州七叶树的子叶中纯化25倍的Valyl-tRNA合成酶。 2.用[^{32 P]焦磷酸盐-ATP交换技术测定时，报告了这三种酶的某些性质。 3.β-（亚甲基环丙基）丙氨酸，异亮氨酸，氮杂亮氨酸，正亮氨酸和γ-羟基降缬氨酸充当亮氨酸-tRNA合成酶的替代底物；该酶对β-（亚甲基环丙基）-丙氨酸和异亮氨酸的亲和力比亮氨酸低约80倍。 4.α-环丙基甘氨酸和α-环丁基甘氨酸充当缬氨酰-tRNA合成酶的替代底物。}

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期刊名称 Biochemical Journal
作者
J. W. Anderson; L. Fowden;
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作者单位

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年(卷),期 1970(119),4
年度 1970
页码 691–697
总页数 7
原文格式 PDF
正文语种
中图分类分子生物学;
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1. Properties and substrate specificity of the leucyl-, the threonyl- and the valyl-transfer-ribonucleic acid synthetases from Aesculus species [J] . J. W. Anderson, L. Fowden The biochemical journal . 1970,第4期

机译：七叶树属物种的亮氨酰基-，苏氨酰基-和戊基转移-核糖核酸合成酶的性质和底物特异性
2. Properties and substrate specificities of the phenylalanyl-transfer-ribonucleic acid synthetases of Aesculus species [J] . J. W. Anderson, L. Fowden The biochemical journal . 1970,第4期

机译：七叶树属物种的苯丙氨酰转移核糖核酸合成酶的性质和底物特异性
3. Efficient procedure for transferring specific human genes into Chinese hamster cell mutants: interspecific transfer of the human genes encoding leucyl- and asparaginyl-tRNA synthetases. [J] . R E Cirullo, S Dana, J J Wasmuth Molecular and Cellular Biology . 1983,第5期

机译：将特定人类基因转移到中国仓鼠细胞突变体中的有效程序：编码亮氨酰和天冬酰胺基-tRNA合成酶的人类基因的种间转移。
4. Species-Specific Differences in the Operational RNA Code for Aminoacylation of tRNATrp and Selection of Bacillus Subtilis Tryptophanyl-tRNA Synthetase Binding Aptamers [C] . 19th tRNA workshop tRNA 2002"" . 2002

机译：种特定差异的操作RNA编码的tRNATrp的氨基酰化和枯草芽孢杆菌色氨酸-tRNA合成酶结合适体的选择。
5. Investigations of the substrate specificity of lacticin 481 synthetase and utilization in peptide engineering applications. [D] . Levengood, Matthew. 2008

机译：乳酸菌素481合成酶的底物特异性研究及其在肽工程应用中的利用。
6. Properties and substrate specificities of the phenylalanyl-transfer-ribonucleic acid synthetases of Aesculus species [O] . J. W. Anderson, L. Fowden 1970

机译：七叶树属物种的苯丙氨酰转移核糖核酸合成酶的性质和底物特异性
7. Properties and substrate specificity of the leucyl-, the threonyl- and the valyl-transfer-ribonucleic acid synthetases from Aesculus species [O] . Anderson, J. W., Fowden, L. 1970

机译：七叶树属物种的亮氨酰基-，苏氨酰基-和戊基转移-核糖核酸合成酶的性质和底物特异性
8. Physiological Properties and Substrate Specificity of a Pentachlorophenol-Degrading 'Pseudomonas' Species [R] . Resnick, S. M., Chapman, P. J. 1994

机译：五氯苯酚降解'假单胞菌'物种的生理特性和底物特异性

Properties and substrate specificity of the leucyl- the threonyl- and the valyl-transfer-ribonucleic acid synthetases from Aesculus species

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