首页> 美国卫生研究院文献>Biochemical Journal >Deoxyribonucleic acid synthesis in mammalian systems. Permealysed Ehrlich ascites cells in vitro first label Okazaki-type low-molecular-weight deoxyribonucleic acid and then high-molecular-weight deoxyribonucleic acid
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Deoxyribonucleic acid synthesis in mammalian systems. Permealysed Ehrlich ascites cells in vitro first label Okazaki-type low-molecular-weight deoxyribonucleic acid and then high-molecular-weight deoxyribonucleic acid

机译:哺乳动物系统中的脱氧核糖核酸合成。体外渗透的Ehrlich腹水细胞首先标记Okazaki型低分子量脱氧核糖核酸然后标记高分子量脱氧核糖核酸

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摘要

During the evaluation of a method of preparing permealysed Ehrlich ascites cells, shortterm labelling experiments were carried out with d[3H]TTP. In the first minute the bulk of the label appeared as low-molecular-weight pieces of DNA. Subsequently the label appeared in DNA of much higher molecular weight. A brief description of the preparation procedure and the properties of the product is provided. Evidence is presented to show that the nucleotide was incorporated directly without intermediate conversion into dTMP or thymidine.
机译:在评估透化的Ehrlich腹水细胞方法的评估过程中,使用d [ 3 H] TTP进行了短期标记实验。在第一分钟,标签的大部分显示为低分子量的DNA。随后,标记出现在分子量更高的DNA中。提供了制备步骤和产品性能的简要说明。提供的证据表明核苷酸被直接掺入而没有中间转化为dTMP或胸苷。

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