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Concentration of MgATP2- and other ions in solution. Calculation of the true concentrations of species present in mixtures of associating ions.

机译:溶液中MgATP2-和其他离子的浓度。计算缔合离子混合物中存在的物质的真实浓度。

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摘要

1. A simple method is described for calculating the free concentrations of all species in a mixture of several ionic components that associate at equilibrium to any extent and with any stoicheiometry. 2. It can readily be adapted to take account of species such as protons for which the free rather than the total concentrations are controlled. 3. It was applied to mixtures of adenine nucleotides, Mg2+ and other ions relevant to the study of glucokinase (EC 2.7.1.2), but the qualitative conclusions are not peculiar to this system. 4. ATP exists in a high and nearly constant proportion (about 80%) as MgATP2- in solutions in which the total MgCl2 concentration exceeds the total ATP concentration by 1-10 mM. 5. By contrast, the proportion of ATP present as MgATP2- varies greatly if the total MgCl2 and total ATP concentrations are varied in constant proportion.
机译:1.一种简单的方法,用于计算几种离子组分的混合物中所有物种的自由浓度,这些离子组分在任何程度上均以平衡和化学计量关系缔合。 2.可以很容易地调整它,以考虑诸如质子之类的受其自由浓度而非总浓度控制的物种。 3.它被用于腺嘌呤核苷酸,Mg2 +和其他与葡萄糖激酶研究有关的离子的混合物(EC 2.7.1.2),但定性结论对该系统而言并不特殊。 4.在总MgCl2浓度超过总ATP浓度1-10 mM的溶液中,ATP以与MgATP2-几乎相同的比例(约80%)存在。 5.相比之下,如果总MgCl2和总ATP浓度以恒定比例变化,则以MgATP2-形式存在的ATP比例变化很大。

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