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首页> 美国卫生研究院文献>Biochemical Journal >Polypeptide linkages and resulting structural features as powerful chromogenic factors in the Lowry phenol reaction. Studies on a glycoprotein containing no Lowry phenol-reactive amino acids and on its desialylated and deglycosylated products.
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Polypeptide linkages and resulting structural features as powerful chromogenic factors in the Lowry phenol reaction. Studies on a glycoprotein containing no Lowry phenol-reactive amino acids and on its desialylated and deglycosylated products.

机译：多肽键和由此产生的结构特征是Lowry苯酚反应中的强大生色因子。不包含Lowry酚反应性氨基酸的糖蛋白及其去唾液酸化和去糖基化产物的研究。

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摘要

With bovine serum albumin as the reference standard, the armadillo salivary-gland glycoprotein, although containing no chromogenic amino acids and only small amounts of colour-yielding peptides [Chou & Goldstein (1960) Biochem. J. 75, 100-115], is highly reactive in the Lowry phenol protein assay [Wu & Pigman (1977) Biochem. J. 161, 37-47]. After desialylation and Smith degradation of the glycoprotein, the Lowry phenol value increased by 13 and 30% respectively, which suggests that both sialic acid and N-acetylhexosamine exert shielding effects in this reaction. Acid hydrolysis for 30 min decreased the Lowry phenol value by more than 45%, which indicates that the peptide linkages and steric features affect the Lowry phenol reactivity. After hydrolysis for up to 6h, the remaining Lowry phenol value of the partially hydrolysed core protein paralleled the amount of unhydrolysed peptides, inferring that both acid-sensitive and acid-resistant chromophoric peptides are fairly evenly distributed along the whole polypeptide chain. As with bovine serum albumin, more than 80% of the colour yield obtained in the Lowry phenol assay with this glycoprotein is Cu2+-dependent.
机译：以牛血清白蛋白为参考标准，犰狳唾液腺糖蛋白虽然不含发色氨基酸，并且仅含有少量发色肽[Chou＆Goldstein（1960）Biochem。 J. 75，100-115]，在Lowry酚蛋白测定中具有高反应性[Wu＆Pigman（1977）Biochem。 J. 161，37-47]。糖蛋白脱唾液酸化和Smith降解后，Lowry苯酚值分别增加了13％和30％，这表明唾液酸和N-乙酰己糖胺均在该反应中发挥屏蔽作用。酸水解30分钟使Lowry苯酚值降低了45％以上，这表明肽键和空间特征会影响Lowry苯酚的反应性。水解长达6小时后，部分水解核心蛋白的剩余Lowry苯酚值与未水解肽的量平行，这说明酸敏感性和耐酸性发色肽均沿整个多肽链相当均匀地分布。与牛血清白蛋白一样，在Lowry酚测定中使用该糖蛋白获得的颜色得率的80％以上是Cu2 +依赖性的。

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