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首页> 美国卫生研究院文献>Biochemical Journal >Characterization of the cholera toxin receptor on Balb/c 3T3 cells as a ganglioside similar to or identical with ganglioside GM1. No evidence for galactoproteins with receptor activity.
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Characterization of the cholera toxin receptor on Balb/c 3T3 cells as a ganglioside similar to or identical with ganglioside GM1. No evidence for galactoproteins with receptor activity.

机译：Balb / c 3T3细胞上霍乱毒素受体的特征是与神经节苷脂GM1类似或相同的神经节苷脂。没有证据表明半乳糖蛋白具有受体活性。

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Balb/c 3T3 cells contain a large number [(0.8-1.6) x 10(6)] of high-affinity (half-maximal binding at 0.2 nM) binding sites for cholera toxin that are resistant to proteolysis, but are quantitatively extracted with chloroform/methanol. The following evidence rigorously establishes that the receptor is a ganglioside similar to, or identical with, ganglioside GM1 by the galactose oxidase/NaB3H4 technique on intact cells was inhibited by cholera toxin. (2) Ganglioside GM1 was specifically adsorbed from Nonidet P40 extracts of both surface- (galactose oxidase/NaB3H4 technique) and metabolically ([1-14C]palmitate) labelled cells in the presence of cholera toxin, anti-toxin and Staphylococcus aureus. (3) Ganglioside GM1 was the only ganglioside labelled when total cellular gangliosides separated on silica-gel sheets were overlayed with 125I-labelled cholera toxin, although GM3 and GD1a were the major gangliosides present. In contrast no evidence for a galactoprotein with receptor activity was obtained. Cholera toxin did not protect the terminal galactose residues of cell-surface glycoproteins from labelling by the galactose oxidase/NaB3H4 technique. No toxin-binding proteins could be identified in Nonidet P40 extracts of [35S]-methionine-labelled cells by immunochemical means. After sodium dodecyl sulphate/polyacrylamide-gel electrophoresis none of the major cellular galactoproteins identified by overlaying gels with 125I-labelled ricin were able to bind 125I-labelled cholera toxin. It is concluded that the cholera toxin receptor on Balb/c 3T3 cells is exclusively ganglioside GM1 (or a related species), and that cholera toxin can therefore be used to probe the function and organisation of gangliosides in these cells as previously outlined [Critchley, Ansell, Perkins, Dilks & Ingram (1979) J. Supramol. Struct. 12, 273-291].
机译：Balb / c 3T3细胞包含大量[（0.8-1.6）x 10（6）]与霍乱毒素的高亲和力结合位点（在0.2 nM处有一半最大结合），这些结合位点对蛋白水解有抵抗力，但可以用氯仿/甲醇。以下证据严格地证明，通过半乳糖氧化酶/ NaB3H4技术，该受体是一种与神经节苷脂GM1相似或相同的神经节苷脂，其对完整细胞的抑制作用是由霍乱毒素抑制的。 （2）在存在霍乱毒素，抗毒素和金黄色葡萄球菌的情况下，从表面（半乳糖氧化酶/ NaB3H4技术）和代谢性（[1-14C]棕榈酸）标记的细胞的Nonidet P40提取物中特异性吸附神经节苷脂GM1。 （3）当在硅胶板上分离的总细胞神经节苷脂上覆盖有125 I标记的霍乱毒素时，神经节苷脂GM1是唯一的神经节苷脂标记物，尽管GM3和GD1a是主要的神经节苷脂。相反，未获得具有受体活性的半乳蛋白的证据。霍乱毒素不能保护细胞表面糖蛋白的末端半乳糖残基不被半乳糖氧化酶/ NaB3H4技术标记。通过免疫化学方法无法在[35S]-蛋氨酸标记的细胞的Nonidet P40提取物中鉴定出毒素结合蛋白。十二烷基硫酸钠/聚丙烯酰胺凝胶电泳后，通过用125 I标记的蓖麻蛋白覆盖凝胶鉴定出的主要细胞半乳糖蛋白均不能结合125 I标记的霍乱毒素。结论是，Balb / c 3T3细胞上的霍乱毒素受体仅是神经节苷脂GM1（或相关物种），因此霍乱毒素可用于探查这些细胞中神经节苷脂的功能和组织，如前所述[Critchley， Ansell，Perkins，Dilks＆Ingram（1979）J. Supramol。结构。 12，273-291]。

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