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首页> 美国卫生研究院文献>Biochemical Journal >Fluorescence quenching of human orosomucoid. Accessibility to drugs and small quenching agents.
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Fluorescence quenching of human orosomucoid. Accessibility to drugs and small quenching agents.

机译：人类类固醇的荧光猝灭。可及药物和小型淬灭剂。

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摘要

The fluorescence behaviour of human orosomucoid was investigated. The intrinsic fluorescence was more accessible to acrylamide than to the slightly larger succinimide, indicating limited accessibility to part of the tryptophan population. Although I- showed almost no quenching, that of Cs+ was enhanced, and suggested a region of negative charge proximal to an emitting tryptophan residue. Removal of more than 90% of sialic acid from the glycan chains led to no change in the Cs+, I-, succinimide or acrylamide quenching, indicating that the negatively charged region originates with the protein core. Quenching as a function of pH and temperature supported this view. The binding of chlorpromazine monitored by fluorescence quenching, in the presence and in the absence of the small quenching probes (above), led to a model of its binding domain on orosomucoid that includes two tryptophan residues relatively shielded from the bulk solvent, with the third tryptophan residue being on the periphery of the domain, or affected allotopically and near the negatively charged field.
机译：研究了人类类固醇的荧光行为。内酰胺荧光比稍大的琥珀酰亚胺更易接近丙烯酰胺，表明对部分色氨酸种群的可及性有限。尽管I-几乎没有淬灭，但Cs +的淬灭却增强了，并暗示了靠近发射色氨酸残基的负电荷区域。从聚糖链中去除超过90％的唾液酸不会导致Cs +，I-，琥珀酰亚胺或丙烯酰胺猝灭的变化，表明带负电荷的区域起源于蛋白核心。淬火与pH和温度的关系证明了这一观点。在存在和不存在小型猝灭探针的情况下，通过荧光猝灭监测氯丙嗪的结合（上图），导致了其在类球蛋白上的结合域模型，该模型包括相对于大部分溶剂相对屏蔽的两个色氨酸残基，第三个色氨酸残基在结构域的外围，或异位地且在带负电荷的区域附近受影响。

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