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美国卫生研究院文献>Biochemical Journal
>The glycoprotein nature of pig kidney diamine oxidase. Role of disulphide groups and arginine residues in the concanavalin A-diamine oxidase interaction.
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The glycoprotein nature of pig kidney diamine oxidase. Role of disulphide groups and arginine residues in the concanavalin A-diamine oxidase interaction.
Pig kidney diamine oxidase (DAO) was found to contain 5% (w/w) natural hexose, 3.25% glucosamine, 2.61% N-acetylglucosamine and 0.25% N-acetylneuraminic acid. The enzyme exhibited strong affinity towards concanavalin A (Con A) with a stoichiometry of 1:4.6. The kinetics of interaction approached an apparent first-order rate, with a rate constant (Kapp.) value of 1.5 x 10(-2) min-1. The enzyme reduced with dithiothreitol followed by alkylation with iodoacetamide showed an increase in the stoichiometry of the Con A-DAO interaction. Similarly arginine modification by phenylglyoxal caused decreased affinity, with an altered Kapp. value of 9.09 x 10(-3) min-1. The results suggest that, besides the carbohydrate content, the protein moiety of the enzyme also plays a significant role in the Con A-DAO interaction.
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机译：发现猪肾脏二胺氧化酶（DAO）包含5％（w / w）的天然己糖，3.25％的葡萄糖胺，2.61％的N-乙酰氨基葡萄糖和0.25％的N-乙酰神经氨酸。该酶对伴刀豆球蛋白A（Con A）表现出强亲和力，化学计量比为1：4.6。相互作用动力学接近表观的一级速率，速率常数（Kapp。）值为1.5 x 10（-2）min-1。用二硫苏糖醇还原的酶，然后用碘乙酰胺烷基化，表明Con A-DAO相互作用的化学计量增加。同样，苯乙二醛对精氨酸的修饰会导致亲和力下降，Kapp改变。值为9.09 x 10（-3）min-1。结果表明，除碳水化合物含量外，酶的蛋白质部分在Con A-DAO相互作用中也起着重要作用。
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