首页> 美国卫生研究院文献>Biochemical Journal >Partial inhibition by cyclosporin A of the swelling of liver mitochondria in vivo and in vitro induced by sub-micromolar Ca2+ but not by butyrate. Evidence for two distinct swelling mechanisms.

【2h】

Partial inhibition by cyclosporin A of the swelling of liver mitochondria in vivo and in vitro induced by sub-micromolar Ca2+ but not by butyrate. Evidence for two distinct swelling mechanisms.

机译：亚微摩尔Ca2 +引起的环孢菌素A部分抑制体内和体外肝脏线粒体的肿胀但不引起丁酸盐的抑制。有两种不同的溶胀机制的证据。

代理获取

本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。

页面导航

摘要
著录项
相似文献
相关主题

摘要

1. The effects of cyclosporin A on the increase in matrix PPi and consequent swelling of energized liver mitochondria incubated with 1 mM-butyrate, 30 microM-bongkrekic acid or 0.1-35 microM-Ca2+ [Halestrap (1989) Biochim. Biophys. Acta 973, 355-382] were studied. 2. Cyclosporin (1 microM) had no significant effect on the swelling induced by butyrate, bongkrekic acid or Ca2+ at concentrations of less than 0.3 microM. 3. At higher [Ca2+] (greater than 0.3 microM), swelling became progressively inhibited by cyclosporin, although the increase in matrix PPi was slightly greater in the presence than in the absence of cyclosporin. 4. Titration with cyclosporin indicated that there are 128 pmol of relevant cyclosporin-binding sites per mg of mitochondrial protein, with a Ki of about 5 nM. 5. The decrease in light-scattering by hepatocytes induced by butyrate [Davidson & Halestrap (1988) Biochem. J. 254, 379-384] was unaffected by cyclosporin, whereas that induced by vasopressin was inhibited by 20-30% without a significant change in cellular PPi content. 6. It is suggested that there are two mechanisms for the increase in mitochondrial volume induced by Ca2+: a PPi-mediated mechanism that is insensitive to cyclosporin and an additional Ca2(+)-mediated effect that is inhibited by cyclosporin. The nature of these pathways and their inter-relationship is discussed in the following paper [Halestrap & Davidson (1990) Biochem. J. 268, 153-160].

机译：1.环孢菌素A对基质PPi的增加以及随后与1 mM丁酸酯，30 microM奉克酸或0.1-35 microM-Ca2 +孵育的充满活力的肝线粒体肿胀的影响[Halestrap（1989）Biochim。生物物理学。研究学报973，355-382]。 2.环孢菌素（1 microM）对丁酸，邦克雷酸或Ca2 +浓度小于0.3 microM引起的肿胀没有明显影响。 3.在较高的[Ca2 +]（大于0.3 microM）下，环孢菌素逐渐溶胀，尽管存在时基质PPi的增加比不存在环孢菌素时的增加。 4.用环孢菌素滴定表明，每毫克线粒体蛋白有128 pmol相关环孢菌素结合位点，Ki约为5 nM。 5.由丁酸盐诱导的肝细胞光散射的减少[Davidson＆Halestrap（1988）Biochem。 J. 254，379-384]不受环孢菌素的影响，而由加压素诱导的抑制作用被抑制20-30％，而细胞PPi含量没有明显变化。 6.建议存在由Ca2 +引起的线粒体体积增加的两种机制：对环孢菌素不敏感的PPi介导的机制和由环孢菌素抑制的另一种Ca2（+）介导的作用。这些途径的性质及其相互关系在以下论文中讨论[Halestrap＆Davidson（1990）Biochem。 J. 268，153-160]。

著录项

期刊名称 Biochemical Journal
作者
A M Davidson; A P Halestrap;
展开▼
作者单位

展开▼
年(卷),期 1990(268),1
年度 1990
页码 147–152
总页数 6
原文格式 PDF
正文语种
中图分类分子生物学;
关键词

相似文献

外文文献
中文文献
专利

1. Partial inhibition by cyclosporin A of the swelling of liver mitochondria in vivo and in vitro induced by sub-micromolar [Ca2+], but not by butyrate. Evidence for two distinct swelling mechanisms [J] . A M Davidson, A P Halestrap The biochemical journal . 1990,第1期

机译：亚微摩尔[Ca2 +]引起的环孢菌素A对体内和体外肝线粒体肿胀的部分抑制作用，但不由丁酸盐引起。两种不同溶胀机制的证据
2. Inhibition of Ca2+-induced large-amplitude swelling of liver and heart mitochondria by cyclosporin is probably caused by the inhibitor binding to mitochondrial-matrix peptidyl-prolyl cis-trans isomerase and preventing it interacting with the adenine nucleotide translocase [J] . A M Davidson, A P Halestrap The biochemical journal . 1990,第1期

机译：环孢菌素对Ca2 +诱导的肝和心脏线粒体大幅度肿胀的抑制作用可能是由于抑制剂与线粒体基质肽-脯氨酰顺反异构酶结合并阻止其与腺嘌呤核苷酸转位酶相互作用
3. Inhibition by secalonic acid D of oxidative phosphorylation and Ca2+-induced swelling in mitochondria isolated from rat livers. [J] . K Kawai, T Nakamaru, Y Maebayashi, Applied and Environmental Microbiology . 1983,第4期

机译：乙醛酸D抑制从大鼠肝脏分离的线粒体中的氧化磷酸化和Ca2 +诱导的肿胀。
4. Differing Effects of Cyclosporin A on Swelling Amplitude and Time Constant of Mitochondria from Normal and Ischemic Rat Brain [C] . Li-Ping Wu, Fang Shen, Yuan Lu, . 2005

机译：环孢菌素A对正常和缺血大鼠脑线粒体肿胀幅度和时间常数的不同影响
5. Inhibition of Ca2(+)-induced large-amplitude swelling of liver and heart mitochondria by cyclosporin is probably caused by the inhibitor binding to mitochondrial-matrix peptidyl-prolyl cis-trans isomerase and preventing it interacting with the adenine nucleotide translocase. [O] . A P Halestrap, A M Davidson 1990

机译：环孢菌素对Ca2（+）诱导的肝和心脏线粒体大幅度肿胀的抑制作用可能是由于抑制剂与线粒体-基质肽-脯氨酰顺反异构酶结合并阻止其与腺嘌呤核苷酸转位酶相互作用而引起的。
6. Partial inhibition by cyclosporin A of the swelling of liver mitochondria in vivo and in vitro induced by sub-micromolar [Ca2+], but not by butyrate. Evidence for two distinct swelling mechanisms. [O] . Davidson, A M, Halestrap, A P 1990

机译：亚微摩尔[Ca2 +]引起的环孢菌素A部分抑制体内和体外肝脏线粒体的肿胀，但不引起丁酸盐的抑制。有两种不同的溶胀机制的证据。
7. Stoichiometric Relationships in CortisolInduced Swelling of Rat Liver Mitochondria [R] . DeVenuto, F., Westphal, U. 1965

机译：皮质醇的化学计量关系诱导大鼠肝线粒体肿胀

Partial inhibition by cyclosporin A of the swelling of liver mitochondria in vivo and in vitro induced by sub-micromolar Ca2+ but not by butyrate. Evidence for two distinct swelling mechanisms.

摘要

著录项

相似文献

相关主题

期刊订阅