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首页> 美国卫生研究院文献>Biochemical Journal >Heterogeneity of pig lysosomal acid alpha-glucosidase. Affinity to Sephacryl S-200 gel and tissue distribution.
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Heterogeneity of pig lysosomal acid alpha-glucosidase. Affinity to Sephacryl S-200 gel and tissue distribution.

机译：猪溶酶体酸α-葡萄糖苷酶的异质性。对Sephacryl S-200凝胶和组织分布的亲和力。

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摘要

Acid alpha-glucosidase purified from pig liver showed heterogeneity in its affinity to Sephacryl S-200 gel. Acid alpha-glucosidase was separated into two fractions (S1 and S2) by Sephacryl S-200 affinity chromatography. Each fraction contained components at apparent 76 kDa and 67 kDa on SDS/PAGE. The amount of S1 fraction was about 1.3 times that of the S2 fraction. In the kidney the ratio of S1 to S2 fraction was similar to that in the liver. However, the heart contained 1.3 times as much S2 fraction as S1 fraction. The spleen acid alpha-glucosidase consisted mainly of S1 fraction, containing only a 76 kDa component. Immunohistochemically, acid alpha-glucosidase was demonstrated in the macrophages of the spleen. Thus the 76 kDa component in the spleen must come mainly from the macrophages. Lectin-binding analysis was carried out on the components present in the S1 and S2 fractions after electrophoresis and transfer to nitrocellulose sheets. Slight differences in binding observed suggest differences in the structure of the sugar side chains.
机译：从猪肝中纯化的酸性α-葡萄糖苷酶对Sephacryl S-200凝胶的亲和力显示出异质性。通过Sephacryl S-200亲和色谱将酸性α-葡萄糖苷酶分为两部分（S1和S2）。在SDS / PAGE上，每个级分包含表观76 kDa和67 kDa的组分。 S1馏分的量是S2馏分的约1.3倍。在肾脏中，S1与S2的比例与肝脏相似。但是，心脏中的S2分数是S1分数的1.3倍。脾酸α-葡糖苷酶主要由S1部分组成，仅含有76kDa的成分。在免疫组织化学上，在脾的巨噬细胞中证实了酸性α-葡萄糖苷酶。因此，脾脏中的76 kDa成分必须主要来自巨噬细胞。电泳后，将S1和S2馏分中存在的成分进行凝集素结合分析，并将其转移至硝化纤维素片上。观察到的结合的轻微差异表明糖侧链的结构有所不同。

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