首页> 美国卫生研究院文献>Biochemical Journal >Investigation of the subcellular location of the tetrapyrrole-biosynthesis enzyme coproporphyrinogen oxidase in higher plants.

【2h】

Investigation of the subcellular location of the tetrapyrrole-biosynthesis enzyme coproporphyrinogen oxidase in higher plants.

机译：研究高等植物中四吡咯生物合成酶原卟啉原氧化酶的亚细胞位置。

代理获取

本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。

页面导航

摘要
著录项
相似文献
相关主题

摘要

The subcellular location of two enzymes in the biosynthetic pathway for protoporphyrin IX, coproporphyrinogen (coprogen) oxidase (EC 1.3.3.3) and protoporphyrinogen (protogen) oxidase (EC 1.3.3.4) has been investigated in etiolated pea (Pisum sativum) leaves and spadices of cuckoo-pint (Arum maculatum). Plant tissue homogenized in isotonic buffer was subjected to subcellular fractionation to prepare mitochondria and plastids essentially free of contamination by other cellular organelles, as determined by marker enzymes. Protogen oxidase activity measured fluorimetrically was reproducibly found in both mitochondria and etioplasts. In contrast, coprogen oxidase could be detected only in etioplasts, using either a coupled fluorimetric assay or a sensitive radiochemical method. The implications of these results for the synthesis of mitochondrial haem in plants is discussed.

机译：已经在黄化豌豆（Pisum sativum）叶片和小鳞片中研究了原卟啉IX生物合成途径中两种酶的亚细胞位置：原卟啉原（coprogen）氧化酶（EC 1.3.3.3）和原卟啉原（原）氧化酶（EC 1.3.3.4）。杜鹃（Arum maculatum）。将等渗缓冲液中均质化的植物组织进行亚细胞分级分离，以制备线粒体和质体，该质体和质体基本上不受其他细胞器污染，如通过标记酶测定的。在线粒体和质体中均发现了用荧光法测得的原氧化酶活性。相反，使用耦合荧光测定法或灵敏的放射化学方法只能在原生质体中检测到辅酶氧化酶。讨论了这些结果对植物线粒体血红素合成的影响。

著录项

期刊名称 Biochemical Journal
作者
A G Smith; O Marsh; G H Elder;
展开▼
作者单位

展开▼
年(卷),期 1993(292),Pt 2
年度 1993
页码 503–508
总页数 6
原文格式 PDF
正文语种
中图分类分子生物学;
关键词

相似文献

外文文献
中文文献
专利

1. Investigation of the subcellular location of the tetrapyrrole-biosynthesis enzyme coproporphyrinogen oxidase in higher plants [J] . A G Smith, G H Elder, O Marsh The biochemical journal . 1993,第2期

机译：高等植物中四吡咯生物合成酶原卟啉原氧化酶的亚细胞定位研究
2. A radiochemical method for the measurement of coproporphyrinogen oxidase and the utilization of substrates other than coproporphyrinogen III by the enzyme from rat liver [J] . G H Elder, J O Evans The biochemical journal . 1978,第1期

机译：用放射化学方法测定鼠肝中原卟啉原氧化酶和利用除原卟啉原III以外的底物的方法
3. Hexachlorobenzene treatment on hepatic mitochondrial function parameters and intracellular coproporphyrinogen oxidase location. [J] . Sopena YE, Ferramola de Sancovich AM, Sancovich HA International journal of toxicology . 2008,第6期

机译：六氯苯处理对肝线粒体功能参数和细胞内原卟啉原氧化酶位置的影响。
4. Mechanism of enhanced responses after combination photodynamic therapy (cPDT) in carcinoma cells involves C/EBP-mediated transcriptional upregulation of the coproporphyrinogen oxidase (CPO) gene [C] . Sanjay Anand, Tayyaba Hasan, Edward V. Maytin SPIE Conference on Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy . 2013

机译：癌细胞组合光动力治疗（CPDT）后增强响应的机制涉及C / EBP介导的副卟啉氧化酶（CPO）基因的转录上调
5. Coproporphyrinogen oxidase (CPOX) polymorphism in human mercury neurotoxicity. [D] . Li, Tingting. 2009

机译：卟啉原原氧化酶（CPOX）基因多态性对人类汞的神经毒性。
6. A radiochemical method for the measurement of coproporphyrinogen oxidase and the utilization of substrates other than coproporphyrinogen III by the enzyme from rat liver. [O] . G H Elder, J O Evans 1978

机译：一种放射化学方法用于测定鼠原卟啉原氧化酶和利用鼠肝中的酶利用除原卟啉原III以外的底物。
7. Investigation of the subcellular location of the tetrapyrrole-biosynthesis enzyme coproporphyrinogen oxidase in higher plants. [O] . Smith, A G, Marsh, O, Elder, G H 1993

机译：研究高等植物中四吡咯生物合成酶原卟啉原氧化酶的亚细胞位置。

Investigation of the subcellular location of the tetrapyrrole-biosynthesis enzyme coproporphyrinogen oxidase in higher plants.

摘要

著录项

相似文献

相关主题

期刊订阅