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首页> 美国卫生研究院文献>Biochemical Journal >Reactions of nitric oxide with mitochondrial cytochrome c: a novel mechanism for the formation of nitroxyl anion and peroxynitrite.
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Reactions of nitric oxide with mitochondrial cytochrome c: a novel mechanism for the formation of nitroxyl anion and peroxynitrite.

机译：一氧化氮与线粒体细胞色素c的反应：形成亚硝酸根阴离子和过氧亚硝酸盐的新机制。

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The aerobic reactions of nitric oxide with cytochrome c were analysed. Nitric oxide (NO) reacts with ferrocytochrome c at a rate of 200 M-1 s-1 to form ferricytochrome c and nitroxyl anion (NO-). Ferricytochrome c was detected by optical spectroscopy; NO- was detected by trapping with metmyoglobin (Mb3+) to form the EPR-detectable Mb-nitrosyl complex, and by the formation of dimers in yeast ferrocytochrome c via cross-linking of the free cysteine residue. The NO- formed subsequently reacted with oxygen to form peroxynitrite, as measured by the oxidation of dihydrorhodamine 123. NO binds to ferricytochrome c to form the ferricytochrome c-NO complex. The on-rate for this reaction is 1.3+/-0.4x10(3) M-1.s-1, and the off-rate is 0.087+/-0.054 s-1. The dissociation constant (Kd) of the complex is 22+/-7 microM. These reactions of NO with cytochrome c are likely to be relevant to mitochondrial metabolism of NO. Ferricytochrome c can act as a reversible sink for excess NO in the mitochondria. The reduction of NO to NO- by ferrocytochrome c may play a role in the irreversible inhibition of mitochondrial oxygen consumption by peroxynitrite. It is generally assumed that peroxynitrite would be formed in mitochondria via the reaction of NO with superoxide. The finding that NO- is formed from the reaction of NO and ferrocytochrome c provides a means of producing peroxynitrite in the absence of superoxide, via the reaction of NO- with oxygen.
机译：分析一氧化氮与细胞色素c的需氧反应。一氧化氮（NO）与铁细胞色素c以200 M-1 s-1的速率反应，形成铁细胞色素c和亚硝酰阴离子（NO-）。用光谱学方法检测铁色素c。通过捕获铁血红蛋白（Mb3 +）形成EPR可检测的Mb-亚硝酰基复合物，并通过游离半胱氨酸残基的交联在酵母铁细胞色素c中形成二聚体来检测NO-。如通过二氢罗丹明123的氧化所测量的，随后形成的NO-与氧反应形成过亚硝酸盐。NO与亚铁色素c结合形成亚铁色素c-NO配合物。该反应的开启速度为1.3 +/- 0.4x10（3）M-1.s-1，关闭速度为0.087 +/- 0.054 s-1。复合物的解离常数（Kd）为22 +/- 7 microM。 NO与细胞色素c的这些反应可能与NO的线粒体代谢有关。亚铁色素c可以充当线粒体中过量NO的可逆吸收剂。亚铁细胞色素c将NO还原为NO-可能在过氧亚硝酸盐不可逆地抑制线粒体耗氧量中起作用。通常认为过氧化物亚硝酸盐将通过NO与超氧化物的反应在线粒体中形成。由NO与铁细胞色素c的反应形成NO-的发现提供了一种在不存在超氧化物的情况下通过NO-与氧的反应生产过亚硝酸盐的方法。

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