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首页> 美国卫生研究院文献>Avicenna Journal of Medical Biotechnology >Intermittent Feeding Attenuates Clinical Course of Experimental Autoimmune Encephalomyelitis in C57BL/6 Mice
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Intermittent Feeding Attenuates Clinical Course of Experimental Autoimmune Encephalomyelitis in C57BL/6 Mice

机译：间歇喂养可减轻C57BL / 6小鼠实验性自身免疫性脑脊髓炎的临床过程

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Multiple Sclerosis (MS) is an autoimmune inflammatory, demyelinating disease of human central nervous system. Experimental Autoimmune Encephalomyelitis (EAE) is the commonly used animal model of MS. Calorie restriction has been found to reduce inflammation and autoimmune responses and promote neuroprotection. In this study we evaluated the effects of intermittent feeding protocol of the calorie restriction in a mouse model of EAE. Fifty four female mice (C57BL/6) were used in this study. The animals were divided into two dietary groups: ad libitum (AL) (n = 29) with free access to food and water and intermittent feeding (IF) (n = 25) with access to food on alternate days. After 8 weeks, EAE was induced in animals by immunization with MOG antigen (Hooke labs, Lawrence, MA, USA) subcutaneously. AL and IF groups were then further divided into two groups each: AA (ad libitum until the end of study) (n = 16) and AI (subjected to intermittent feeding regimen after immunization day) (n = 13). The IF group was divided into II (continued intermittent feeding regimen until the end of study) (n = 13) and IA (changed to AL regimen after immunization day) (n = 12). All the animals were behaviorally monitored for 35 days after immunization and observed daily for the signs and severity of disease with EAE scoring scale [0–5] and cumulative disease index (CDI) score. Intermittent feeding significantly reduced the incidence of EAE in IF groups (AI 0%, II 18.5%, IA 22.2%, p < 0.05). In addition, intermittent feeding significantly delayed the onset of EAE in AI group (p < 0.05) and also, intermittent feeding significantly reduced the severity of disease in II and IA groups (AA vs. II, p < 0.05 & AA vs. IA p < 0.05) groups. The CDI was also significantly reduced in intermittent feeding fed groups [AI, II and IA compared to AA group (P < 0.05, <0.01, <0.05 respectively)]. Intermittent feeding regimen protocol of the calorie restriction significantly suppressed EAE incidence, induction, and severity. The results of this study suggest possible role of intermittent feeding in the treatment of Multiple Sclerosis patients.
机译：多发性硬化症（MS）是人类中枢神经系统的一种自身免疫性炎症性脱髓鞘疾病。实验性自身免疫性脑脊髓炎（EAE）是MS的常用动物模型。已经发现热量限制可以减少炎症和自身免疫反应并促进神经保护。在这项研究中，我们评估了EAE小鼠模型中限制卡路里的间歇喂养方案的效果。在这项研究中使用了54只雌性小鼠（C57BL / 6）。将动物分为两个饮食组：随意进食（AL）（n = 29），可自由进食和饮水；间歇喂食（IF）（n = 25），可隔日进食。 8周后，通过皮下用MOG抗原（Hooke labs，Lawrence，MA，USA）免疫在动物中诱导EAE。然后将AL和IF组进一步分为两组：AA（随意服用，直到研究结束）（n = 16）和AI（接受免疫日后的间歇喂养方案）（n = 13）。 IF组分为II组（连续间歇喂养方案，直至研究结束）（n = 13）和IA组（免疫日后改为AL组）（n = 12）。免疫后35天对所有动物进行行为监测，并每天用EAE评分等级[0-5]和累积疾病指数（CDI）评分观察疾病的体征和严重程度。间歇喂养显着降低了IF组的EAE发生率（AI 0％，II 18.5％，IA 22.2％，p <0.05）。此外，间歇喂养明显延迟了AI组的EAE发作（p <0.05），并且间歇喂养显着降低了II和IA组的疾病严重程度（AA vs. II，p <0.05＆AA vs. IA p <0.05）组。间歇喂养组的CDI也显着降低[与AA组相比，AI，II和IA（分别为P <0.05，<0.01，<0.05）]。限制卡路里的间歇喂养方案可以显着抑制EAE的发生，诱导和严重程度。这项研究的结果表明间歇性进食在多发性硬化症患者治疗中的可能作用。

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