Interferences in the Optimization of the MTT Assay for Viability Estimation of Proteus mirabilis

机译：在MTT分析方法优化过程中对干扰物变形杆菌的生存能力进行估计的干扰

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Background:The chromogenic assay based on MTT bioreduction was adapted to Proteus mirabilis viability estimations. We primarily intended to use the assay for the evaluation of novel antimicrobial compounds, including structures with possible permeabilizing activity. Therefore, the influence of basic permeabilizing agents like Triton X-100 and EDTA upon the MTT assay was studied.

机译：背景：基于MTT生物还原的生色测定法适用于奇异变形杆菌的生存能力估计。我们的主要目的是将该测定法用于评估新型抗菌化合物，包括可能具有透化活性的结构。因此，研究了诸如Triton X-100和EDTA之类的碱性增透剂对MTT分析的影响。

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期刊名称 Avicenna Journal of Medical Biotechnology
作者
Ewa Grela; Adam Ząbek; Agnieszka Grabowiecka;
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作者单位

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年(卷),期 2015(7),4
年度 2015
页码 159–167
总页数 9
原文格式 PDF
正文语种
中图分类生物学;
关键词
MTT assay Proteus mirabilis Viability;

机译：MTT分析;奇异变形杆菌;生存力;

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2. Influence of serum and albumin on the in vitro anandamide cytotoxicity toward C6 glioma cells assessed by the MTT cell viability assay: Implications for the methodology of the MTT tests [J] . BilminK., KopczynskaB., GriebP. Folia neuropathologica . 2013,第1期

机译：MTT细胞生存力测定评估血清和白蛋白对C6胶质瘤细胞对体外anandamide细胞毒性的影响：MTT测试方法的含义
3. Influence of serum and albumin on the in vitro anandamide cytotoxicity toward C6 glioma cells assessed by the MTT cell viability assay: implications for the methodology of the MTT tests [J] . Krzysztof Bilmin, Beata Kopczyńska, Pawe? Grieb Folia neuropathologica . 2013,第1期

机译：MTT细胞活力测定评估血清和白蛋白对C6胶质瘤细胞对体外anandamide细胞毒性的影响：对MTT测试方法的影响
4. Proteus mirabilis viability after lithotripsy of struvite calculi [C] . Sabitha Prabakharan, Southwest Texas State Univ., San Marcos, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems X . 2000

机译：鸟粪石碎石碎石术后奇异变形杆菌的生存能力
5. Contributions of the Rcs Phosphorelay and FlhD4C 2 to Swarming Behavior in Proteus mirabilis. [D] . Howery, Kristen E. 2016

机译：Rcs Phosphorelay和FlhD4C 2对奇异变形杆菌的成群行为的贡献。
6. Designing and optimization of an electrochemical substitute for the MTT (3-(45-Dimethylthiazol-2-yl)-25-diphenyltetrazolium bromide) cell viability assay [O] . Mohammad Mazloum-Ardakani, Behnaz Barazesh, Seyed Mohammad Moshtaghioun, -1

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7. Effect of extracellular conditions on diarrheal Proteus mirabilis in vitro: a preliminary study on virulence of Proteus mirabilis from a food poisoning in Shenzhen, China [O] . Z.-L. Gong, F. Bai, D. Ng’ambi, 2018

机译：细胞外条件对体外腹泻Proteus mirabilis的影响：中国深圳食物中毒蛋白质霉菌毒力初步研究

Interferences in the Optimization of the MTT Assay for Viability Estimation of Proteus mirabilis

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